Mouse IL-4

Interleukin 4 (IL-4) is an immunomodulatory cytokine that functions to induce naïve helper T cells to differentiate into type 2 T helper (Th2) cells. Th2 cells subsequently produce more IL-4 in a positive feedback loop. IL-4 also promotes immunoglobulin IgG to IgE isotype switching on B cells. IL-4 binds the IL-4Rα receptor to activate STAT6 signaling.

SKU Select size Price Buy
200-18-5ug Mini: 5 ug $63.00 + -
200-18-20ug Std.: 20 ug $158.00 + -
200-18-100ug Value: 100 ug $410.00 + -
200-18-500ug Midgi: 500 ug $2,016.00 + -
200-18-1mg Mg: 1 mg $3,360.00 + -
200-18AF-5ug Animal-Free Mini: 5 ug $69.00 + -
200-18AF-20ug Animal-Free Std.: 20 ug $174.00 + -
200-18AF-100ug Animal-Free Value: 100 ug $436.00 + -
200-18AF-500ug Animal-Free Midgi: 500 ug $2,142.00 + -
200-18AF-1mg Animal-Free Mg: 1 mg $3,570.00 + -
Alternate Names:  B cell Stimulating Factor, BSF-1, BCDF, BCGF              
Accession Number: P07750
Description:  Interleukin 4 (IL-4) is an immunomodulatory cytokine that functions to induce naïve helper T cells to differentiate into type 2 T helper (Th2) cells. Th2 cells subsequently produce more IL-4 in a positive feedback loop. IL-4 also promotes immunoglobulin IgG to IgE isotype switching on B cells. IL-4 binds the IL-4Rα receptor to activate STAT6 signaling.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 13.7 kDa (121 aa)
AA Sequence:  MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVC RASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNES KSTSLKDFLE SLKSIMQMDY S
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

HT-2 proliferation

≤20 ng/mL; ≥ 5.0 x 10^4 units/mg

 

 **** International Units: The specific activity of Mouse IL-4 is approximately 2.5 x 10^4 IU/μg, which is calibrated against recombinant mouse IL-4 WHO International Standard (NIBSC code: 91/656). 

Preparation and Storage:
Country of Origin:  USA        Shipping:  Room temperature             
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 1111 Lot 0613
SDS Lot 0613 Lot 0217
PDS Animal-Free Lot 0217 Lot 1111
SDS Animal-Free Lot 0613 Animal-Free   
  Lot 0217 Animal-Free  

 

 

 

Papers that use this product:
 
 
 2019 Apr;176(7):938-949. doi: 10.1111/bph.14597. Epub 2019 Mar 14. PMID 30706444

Blockage of sphingosine-1-phosphate receptor 2 attenuates allergic asthma in mice.


 2018 Sep 15;10(9):2859-2867. eCollection 2018. PMID 30323872

Mouse dendritic cell migration in abdominal lymph nodes by intraperitoneal administration.

Wang B1,2Sun C2,3Wang S4,5Shang N2Shangguan J2Figini M2Pan L2,6Zhou K2Ma Q2Procissi D2Velichko Y2,7Yaghmai V2,7Li G1Zhang Z2,7. 
 

 2018 Jan;141(1):189-201.e3. doi: 10.1016/j.jaci.2017.03.045. Epub 2017 May 4. PMID 28479335
 2018 Mar;48(3):288-296. doi: 10.1111/cea.13075. Epub 2018 Jan 22. PMID 29247574

IgE promotes type 2 innate lymphoid cells in murine food allergy.


 2016 Apr 22;5(6):e1173299. doi: 10.1080/2162402X.2016.1173299. eCollection 2016 Jun. PMID 27471652

Murine mesothelioma induces locally-proliferating IL-10(+)TNF-α(+)CD206(-)CX3CR1(+) M3 macrophages that can be selectively depleted by chemotherapy or immunotherapy.

 

 2014 Jun;36(3):9655. doi: 10.1007/s11357-014-9655-y. Epub 2014 Apr 19. PMID 24744051 

IL-2/CD40-activated macrophages rescue age and tumor-induced T cell dysfunction in elderly mice.


 2013 Jun;218(6):844-50. doi: 10.1016/j.imbio.2012.10.003. Epub 2012 Oct 17. PMID 23182714

Role of complement in a murine model of peanut-induced anaphylaxis.

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