Mouse IL-33

Interleukin 33 (IL-33) is a member of the IL-1 cytokine family and is constitutively expressed in smooth muscle and airway epithelial cells.  IL-33 signals through the interleukin 1 receptor-like 1 (IL-1R1) and interleukin-1 receptor accessory protein (IL1RAP) receptors to ativate NF-κB and MAPK signaling pathways.  IL-33 functions to induce type 2 cytokine production in polarized Type 2 helper T (Th2) cells.

SKU Select size Price Buy
200-36-2ug Mini: 2 ug $63.00 + -
200-36-10ug Std.: 10 ug $158.00 + -
200-36-100ug Value: 100 ug $609.00 + -
200-36-500ug Midgi: 500 ug $3,024.00 + -
200-36-1mg Mg: 1 mg $5,040.00 + -
200-36AF-2ug Animal-Free Mini: 2 ug $69.00 + -
200-36AF-10ug Animal-Free Std.: 10 ug $174.00 + -
200-36AF-100ug Animal-Free Value: 100 ug $667.00 + -
200-36AF-500ug Animal-Free Midgi: 500 ug $3,329.00 + -
200-36AF-1mg Animal-Free Mg: 1 mg $5,544.00 + -

Alternate Names:  NP-HEV              

Accession Number: Q8BVZ5
Description:  Interleukin 33 (IL-33) is a member of the IL-1 cytokine family and is constitutively expressed in smooth muscle and airway epithelial cells.  IL-33 signals through the interleukin 1 receptor-like 1 (IL-1R1) and interleukin-1 receptor accessory protein (IL1RAP) receptors to ativate NF-κB and MAPK signaling pathways.  IL-33 functions to induce type 2 cytokine production in polarized Type 2 helper T (Th2) cells.  
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 17.7 kDa (159 aa)
AA Sequence:  MSIQGTSLLT QSPASLSTYN DQSVSFVLEN GCYVINVDDS GKDQEQDQVL LRYYESPCPA SQSGDGVDGK KLMVNMSPIK DTDIWLHAND KDYSVELQRG DVSPPEQAFF VLHKKSSDFV SFECKNLPGT YIGVKDNQLA LVEEKDESCN NIMFKLSKI
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥90%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

D10S cell proliferation

≤2 ng/mL; ≥ 5.0 x 10^5 units/mg

(typical ED50 is < 1 ng/mL)

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0515 Lot 0515
SDS Lot 0515 Animal-Free  
PDS Animal-Free    
SDS Animal-Free    

 

 

Papers that use this product:

 

Journal of Immunol June 3, 2019, doi:10.4049/jimmunol.1801005 PMID 31160535

Lactic Acid Inhibits Lipopolysaccharide-Induced Mast Cell
Function by Limiting Glycolysis and ATP Availability


Heather L. Caslin,* Daniel Abebayehu,† Amina Abdul Qayum,† Tamara T. Haque,†
Marcela T. Taruselli,† Patrick A. Paez,† Neha Pondicherry,† Brian O. Barnstein,†
L. Alexis Hoeferlin,‡ Charles E. Chalfant,‡,x,{,‖ and John J. Ryan†

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