Mouse IL-12

* This item is currently on backorder. 

SKU Select size Price Buy
200-25-2ug Mini: 2 ug $63.00 + -
200-25-10ug Std.: 10 ug $158.00 + -
200-25-100ug Value: 100 ug $1,260.00 + -
200-25-500ug Midgi: 500 ug $4,536.00 + -
200-25-1mg Mg: 1 mg $7,560.00 + -

Alternate Names:  NKSF, CLMF               Accession Number: P43432/P43431
Description:  Interleukin 12 (IL-12) is an inducer of cell-mediated immunity and is produced by dendritic cells, macrophages, and B cells after antigen stimulation. IL-12 functions to promote naïve T cell differentiation into the type 1 T helper cell (Th1) lineage. IL-12 also stimulates the production of interferon gamma (IFN-γ) and tumor necrosis factor alpha (TNF-α) from T cells and natural killer cells. Mouse IL-12 is active on human and mouse cells.
Source:  Genetically modified HEK 293 cells.
Predicted MW: Heterodimer, p35: 21.7 kDa, p40: 35.8 kDa, total: 57.5 kDa (p35: 193 aa, p40: 313 aa, total: 506 aa)
AA Sequence:  p35: RVIPVSGPAR CLSQSRNLLK TTDDMVKTAR EKLKHYSCTA EDIDHEDITR DQTSTLKTCL PLELHKNESC LATRETSSTT RGSCLPPQKT SLMMTLCLGS IYEDLKMYQT EFQAINAALQ NHNHQQIILD KGMLVAIDEL MQSLNHNGET LRQKPPVGEA DPYRVKMKLC ILLHAFSTRV VTINRVMGYL SSA

p40: MWELEKDVYV VEVDWTPDAP GETVNLTCDT PEEDDITWTS DQRHGVIGSG KTLTITVKEF LDAGQYTCHK GGETLSHSHL LLHKKENGIW STEILKNFKN KTFLKCEAPN YSGRFTCSWL VQRNMDLKFN IKSSSSSPDS RAVTCGMASL SAEKVTLDQR DYEKYSVSCQ EDVTCPTAEE TLPIELALEA RQQNKYENYS TSFFIRDIIK PDPPKNLQMK PLKNSQVEVS WEYPDSWSTP HSYFSLKFFV RIQRKKEKMK ETEEGCNQKG AFLVEKTSTE VQCKGGNVCV QAQDRYYNSS CSKWACVPCR VRS
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

Induction of SEAP in HEK-Blue™ IL-12 cells

≤ 20 ng/mL; ≥ 5.0 x 10^4 units/mg

 

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0612 Lot 0612
SDS   Lot 0812

 

Papers that cite this product:
 
 

Nat Commun 2019 Feb 11;10(1):701.  doi: 10.1038/s41467-019-08605-3. PMID 30741923

Hectd3 Promotes Pathogenic Th17 Lineage Through Stat3 Activation and Malt1 Signaling in Neuroinflammation

Cancer Res 2018 Jun 1;78(11):3067-3074.  doi: 10.1158/0008-5472.CAN-17-2153. PMID 29636345

Enhanced Lymphodepletion Is Insufficient to Replace Exogenous IL2 or IL15 Therapy in Augmenting the Efficacy of Adoptively Transferred Effector CD8 + T Cells

 Cancer Immunol Res 2015 Dec;3(12):1364-74.  doi: 10.1158/2326-6066.CIR-15-0087-T. Epub 2015 Aug 21. PMID 26297711
 Sci Transl Med 2015 Oct 28;7(311):311ra170.  doi: 10.1126/scitranslmed.aac8155. PMID 26511507
 Cancer Immunol Immunother 2015 May;64(5):539-49.  doi: 10.1007/s00262-015-1655-y. Epub 2015 Feb 13. PMID 25676709