Mouse IFN-gamma

Interferon gamma (IFN-γ) is a type II interferon that is critical during adaptive and innate immune responses to infection. IFN-γ is produced by T cells and natural killer cells following antigen-specific activation. IFN-γ binds IFN-γ receptors (IFN-γ R1 and IFN-γ R2), which are expressed on most immune cells, to activate the JAK-STAT pathway. IFN-γ-induced signaling increases the expression of class 1 major histocompatibility complex (MHC) molecules. Mouse IFN-γ is not cross-reactive with human IFN-γ.

SKU Select size Price Buy
200-16-20ug Mini: 20 ug $63.00 + -
200-16-100ug Std.: 100 ug $158.00 + -
200-16-250ug Value: 250 ug $363.00 + -
200-16-500ug Midgi: 500 ug $604.00 + -
200-16-1mg Mg: 1 mg $1,050.00 + -
200-16AF-20ug Animal-Free Mini: 20 ug $69.00 + -
200-16AF-100ug Animal-Free Std.: 100 ug $174.00 + -
200-16AF-250ug Animal-Free Value: 250 ug $399.00 + -
200-16AF-500ug Animal-Free Midgi: 500 ug $662.00 + -
200-16AF-1mg Animal-Free Mg: 1 mg $1,155.00 + -

Alternate Names:  Type II interferon, T cell interferon, Immune Interferon, MAF               Accession Number: P01580
Description:  Interferon gamma (IFN-γ) is a type II interferon that is critical during adaptive and innate immune responses to infection. IFN-γ is produced by T cells and natural killer cells following antigen-specific activation. IFN-γ binds IFN-γ receptors (IFN-γ R1 and IFN-γ R2), which are expressed on most immune cells, to activate the JAK-STAT pathway. IFN-γ-induced signaling increases the expression of class 1 major histocompatibility complex (MHC) molecules. Mouse IFN-γ is not cross-reactive with human IFN-γ.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 15.7 kDa (134 aa)
AA Sequence:  MHGTVIESLE SLNNYFNSSG IDVEEKSLFL DIWRNWQKDG DMKILQSQII SFYLRLFEVL KDNQAISNNI SVIESHLITT FFSNSKAKKD AFMSIAKFEV NNPQVQRQAF NELIRVVHQL LPESSLRKRK RSRC
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

Viral CPE assay using EMC virus on L929 cells

≥ 1.0 x 10^7 units/mg

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.  

Expiration Date:                      
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 1107 Lot 0416
SDS Lot 0416 Lot 1107
PDS Animal-Free Lot 0416 Animal-Free  
SDS Animal-Free    

 

Papers that use this product:
 
 
 
 2019 Jul 17;11(501). pii: eaav7816. doi: 10.1126/scitranslmed.aav7816. PMID 31316010

Sensitization to immune checkpoint blockade through activation of a STAT1/NK axis in the tumor microenvironment.

Zemek RM1,2De Jong E3Chin WL2,4,5Schuster IS6,7,8Fear VS2,4Casey TH1,2Forbes C2,4Dart SJ1,2Leslie C9Zaitouny A10,11Small M10,11Boon L12Forrest ARR13Muiri DO13Degli-Esposti MA6,7,8Millward MJ4,5Nowak AK2,4,5Lassmann T3Bosco A3Lake RA1,2Lesterhuis WJ14,2,3.

 

 2017 Dec;102(6):1441-1450. doi: 10.1189/jlb.4A0517-179R. Epub 2017 Sep 26. PMID 28951422

Hematopoietic MyD88 and IL-18 are essential for IFN-γ-dependent restriction of type A Francisella tularensis infection.


 2015 Aug;3(8):946-55. doi: 10.1158/2326-6066.CIR-14-0206. Epub 2015 Jun 3. PMID 26041735

PD-1 or PD-L1 Blockade Restores Antitumor Efficacy Following SSX2 Epitope-Modified DNA Vaccine Immunization.


 2014 Jun;36(3):9655. doi: 10.1007/s11357-014-9655-y. Epub 2014 Apr 19. PMID 24744051 

IL-2/CD40-activated macrophages rescue age and tumor-induced T cell dysfunction in elderly mice.


 2013 Jun;12(3):345-57. doi: 10.1111/acel.12062. Epub 2013 Mar 27. PMID 23442123

Targeting macrophages rescues age-related immune deficiencies in C57BL/6J geriatric mice.


 2010 Aug;89(1):1-8. doi: 10.1016/j.yexmp.2010.04.001. Epub 2010 Apr 28. PMID 20433827

The role of cytokines in UbD promoter regulation and Mallory-Denk body-like aggresomes.


 2010 Jun;88(3):353-62. doi: 10.1016/j.yexmp.2010.03.001. Epub 2010 Mar 16. PMID 20223233

SAMe prevents the induction of the immunoproteasome and preserves the 26S proteasome in the DDC-induced MDB mouse model.

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