Mouse FLT-3 Ligand

 Fms-related tyrosine kinase 3 ligand (FLT-3 ligand) is a growth factor that regulates hematopoietic cell proliferation. FLT-3 ligand signalling is transmitted through the fms-related tyrosine kinase 3 (FLT-3) receptor. FLT-3 ligand promotes the long-term expansion and differentiation of pro-B cells in the presence of interleukin 7 (IL-7,) or in combination of IL-7 and interleukin 3 (IL-3).

SKU Select size Price Buy
200-06-2ug Mini: 2 ug $63.00 + -
200-06-10ug Std.: 10 ug $158.00 + -
200-06-100ug Value: 100 ug $457.00 + -
200-06-500ug Midgi: 500 ug $2,268.00 + -
200-06-1mg Mg: 1 mg $3,780.00 + -
200-06AF-2ug Animal-Free Mini: 2 ug $69.00 + -
200-06AF-10ug Animal-Free Std.: 10 ug $174.00 + -
200-06AF-100ug Animal-Free Value: 100 ug $504.00 + -
200-06AF-500ug Animal-Free Midgi: 500 ug $2,494.00 + -
200-06AF-1mg Animal-Free Mg: 1 mg $4,158.00 + -
Alternate Names:  Flt3L, Fms-related tyrosine kinase 3 ligand               
Accession Number: P49772
Description:  Fms-related tyrosine kinase 3 ligand (FLT-3 ligand) is a growth factor that regulates hematopoietic cell proliferation. FLT-3 ligand signalling is transmitted through the fms-related tyrosine kinase 3 (FLT-3) receptor. FLT-3 ligand promotes the long-term expansion and differentiation of pro-B cells in the presence of interleukin 7 (IL-7,) or in combination of IL-7 and interleukin 3 (IL-3).
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 18.6 kDa (163 aa)
AA Sequence:  MTPDCYFSHS PISSNFKVKF RELTDHLLKD YPVTVAVNLQ DEKHCKALWS LFLAQRWIEQ LKTVAGSKMQ TLLEDVNTEI HFVTSCTFQP LPECLRFVQT NISHLLKDTC TQLLALKPCI GKACQNFSRC LEVQCQPDSS TLLPPRSPIA LEATELPEPR PRQ
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

OCI-AML5 cell proliferation

≤10 ng/mL; ≥ 1.0 x 10^5 units/mg 

 
Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0318 Lot 0318
SDS Lot 0212 Lot 10191
PDS Animal-Free Lot 0318 Animal-Free   
SDS Animal-Free    

 

 

Papers that use this product:
 
 
 2018 Mar 8;9(1):996. doi: 10.1038/s41467-018-03409-3. PMID 29520027
 2017 Aug 28;8:1037. doi: 10.3389/fimmu.2017.01037. eCollection 2017. PMID 28894448 

Sirtuin 2 Deficiency Increases Bacterial Phagocytosis by Macrophages and Protects from Chronic Staphylococcal Infection.


 2017 Jun 20;1(15):1067-1079. doi: 10.1182/bloodadvances.2016002931. eCollection 2017 Jun 27. PMID 29296749

High CD123 levels enhance proliferation in response to IL-3, but reduce chemotaxis by downregulating CXCR4 expression.

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