Email orders to

Human M-CSF

Human MCSF, Macrophage Colony Stimulating Factor, is a recombinant protein used in cell culture to differentiate primary PBMCs & monocytes to macrophages. Tested for biological activity in M-NFS-60 cell proliferation assay. Lyophilized protein, high purity, low endotoxin & high quality M-CSF manufactured in the USA by protein manufacturer Shenandoah.

SKU Select size Price Buy
100-03-2ug Mini: 2 ug $65.00 + -
100-03-10ug Std.: 10 ug $163.00 + -
100-03-100ug Value: 100 ug $524.00 + -
100-03-500ug Midgi: 500 ug $2,531.00 + -
100-03-1mg Mg: 1 mg $4,218.00 + -
100-03AF-2ug Animal-Free Mini: 2 ug $70.00 + -
100-03AF-10ug Animal-Free Std.: 10 ug $178.00 + -
100-03AF-100ug Animal-Free Value: 100 ug $579.00 + -
100-03AF-500ug Animal-Free Midgi: 500 ug $2,785.00 + -
100-03AF-1mg Animal-Free Mg: 1 mg $4,640.00 + -

Macrophage colony stimulating factor (M-CSF) is a hematopoietic growth factor that is widely produced by a variety of cells. M-CSF stimulates the proliferation and differentiation of hematopoietic stem cells into monocyte and macrophage cell types. M-CSF also acts through the colony stimulating factor 1 receptor (CSF1R) to modulate processes involved in immunology, bone metabolism, fertility, and pregnancy. Human M-CSF shows activity on mouse cells; however, mouse M-CSF shows no activity on human cells.
Source:  Genetically modified E.coli.

Catalog Number: 100-03

Alternate Names:  MGI-IM, CSF-1               Accession Number: P09603

Predicted MW: Dimer, 18.5/37.1 kDa (159/318 aa)
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 100 mM sodium chloride, pH 8.0

M-CSF Product Specifications*

*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 


Method of Determination

Acceptance Criteria


Reducing and Non-Reducing SDS PAGE



Kinetic LAL

≤1 EUs/µg

Biological Activity

NFS-60 cell proliferation

≤10 ng/mL; ≥ 1.0 x 10^5 units/mg


 *** International Units: The specific activity of Human M-CSF is 1.0 x 10^5
 IU/μg, which is calibrated against recombinant human M-CSF WHO International Standard (NIBSC code: 89/512).

Preparation and Storage

Country of Origin:  USA       

Shipping:  Room temperature             Storage Prior to Reconstitution:  -20°C

Product Reconstitution:  Sterile water at 0.1 mg/mL

Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date

12 months from date of receipt when stored at -20°C to-80°C as supplied.

1 month when stored at 4°C after reconstituting as directed.

3 months when stored at -20°C to -80°C after reconstituting as directed.


To receive more information or for a bulk quote, email


Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0311 Lot 0114
SDS Lot 1008 Lot 1117
PDS Animal-Free Lot 1117  
SDS Animal-Free Lot 1117 Animal-Free  
 Papers that cite this product:

Differentiated Agonistic Antibody Targeting CD137 Eradicates Large Tumors Without Hepatotoxicity

Genes and Immunity 2019 Sep;20(7):577-588.  doi: 10.1038/s41435-019-0059-y. Epub 2019 Jan 29. PMID: 30692607
 2019 Feb 15;202(4):1163-1175. doi: 10.4049/jimmunol.1801053. Epub 2019 Jan 11. PMID 30635391

Leishmania donovani Induces Autophagy in Human Blood-Derived Neutrophils.

 2018 Sep;99(9):1239-1247. doi: 10.1099/jgv.0.001119. Epub 2018 Jul 30. PMID  30058991

iPS cell serves as a source of dendritic cells for in vitro dengue virus infection model.

 2016 Jul 27;5. pii: e13024. doi: 10.7554/eLife.13024. PMID 27462873

Tissue damage drives co-localization of NF-κB, Smad3, and Nrf2 to direct Rev-erb sensitive wound repair in mouse macrophages.

 2018 Jan;106:194-203. doi: 10.1016/j.bone.2017.09.020. Epub 2017 Sep 28. PMID 28965919

Pulsed electromagnetic fields inhibit human osteoclast formation and gene expression via osteoblasts.


 2018 Feb;515:176-190. doi: 10.1016/j.virol.2017.12.025. Epub 2018 Jan 2. PMID 29304470

Protein S and Gas6 induce efferocytosis of HIV-1-infected cells.

 2017 Jun 1;127(6):2192-2205. doi: 10.1172/JCI86282. Epub 2017 Apr 24. PMID 28436939

An inflammatory bowel disease-risk variant in INAVA decreases pattern recognition receptor-induced outcomes.

 2017 Dec;35(12):2658-2666. doi: 10.1002/jor.23587. Epub 2017 Jul 18. PMID 28460421

Calreticulin inhibits inflammation-induced osteoclastogenesis and bone resorption.


 2015 Jul 15;24(14):4147-57. doi: 10.1093/hmg/ddv142. Epub 2015 May 1. PMID 25935003

Improved integrative framework combining association data with gene expression features to prioritize Crohn's disease genes.

 2013 Jan 15;73(2):662-71. doi: 10.1158/0008-5472.CAN-12-0653. PMID 23329645

Related products