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Human EGF

Epidermal growth factor (EGF) is a growth factor that stimulates the proliferation, differentiation, and survival of epithelial and epidermal cells. EGF contains three intramolecular disulfide bonds and binds in high affinity to the epidermal growth factor receptor (EGFR). EGF is overexpressed in multiple tumor cell lines and promotes resistance to chemotherapy and radiation treatments.

 

 

SKU Select size Price Buy
100-26-100ug Mini: 100 ug $65.00 + -
100-26-500ug Std.: 500 ug $163.00 + -
100-26-1mg Value: 1 mg $238.00 + -
100-26-5mg Mg: 5 mg $1,082.00 + -
100-26AF-100ug Animal-Free Mini: 100 ug $70.00 + -
100-26AF-500ug Animal-Free Std.: 500 ug $178.00 + -
100-26AF-1mg Animal-Free Value: 1 mg $265.00 + -
100-26AF-5mg Animal-Free Mg: 5 mg $1,190.00 + -

Alternate Names:  Urogastrone, URG               Accession Number: P01133
Description:  Epidermal growth factor (EGF) is a growth factor that stimulates the proliferation, differentiation, and survival of epithelial and epidermal cells. EGF contains three intramolecular disulfide bonds and binds in high affinity to the epidermal growth factor receptor (EGFR). EGF is overexpressed in multiple tumor cell lines and promotes resistance to chemotherapy and radiation treatments.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 6.2 kDa (53 aa)
AA Sequence:  NSDSECPLSH DGYCLHDGVC MYIEALDKYA CNCVVGYIGE RCQYRDLKWW ELR
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

3T3 cell proliferation

≤100 pg/mL

 **** International Units: The specific activity of Human EGF is approximately 1.3 x 10^3 IU/μg, which is calibrated against recombinant human EGF WHO International Standard (NIBSC code: 91/530). 

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0712 Lot 0216
SDS Lot 0216  
PDS Animal-Free Lot 0213 Animal-Free  
SDS Animal-Free  Lot 0216 Animal-Free  

 

Papers that use this product:
 
 
July 2020 UC Santa Cruz. ProQuest ID: Sanders_ucsc_0036E_12024. Merritt ID: ark:/13030/m5102czj. Retrieved from https://escholarship.org/uc/item/8452n2gj
 
 
Sanders, L. M. (2020)
Biotech Bioeng. 2020 Feb;117(2):511-522.  doi: 10.1002/bit.27219. Epub 2019 Nov 19. PMID 31691953

Three-dimensional Biomimetic Hyaluronic Acid Hydrogels to Investigate Glioblastoma Stem Cell Behaviors


 2019 Jun 26;8(7). pii: E911. doi: 10.3390/jcm8070911. PMID 31247884

 2019 Apr;12(1):47-56. doi: 10.1007/s12307-019-00224-2. Epub 2019 May 11. PMID 31079324

Targeting Hyaluronan Interactions for Glioblastoma Stem Cell Therapy.


 2019 Mar 4;10(1):1023. doi: 10.1038/s41467-019-08823-9. PMID 30833574

ACVR1 R206H cooperates with H3.1K27M in promoting diffuse intrinsic pontine glioma pathogenesis.


 2019 Jan 2;5(1):eaat0456. doi: 10.1126/sciadv.aat0456. eCollection 2019 Jan. PMID 30613765 

IDH3α regulates one-carbon metabolism in glioblastoma.


 2019 Jan 10;4(1). pii: 122933. doi: 10.1172/jci.insight.122933. [Epub ahead of print] PMID 30626740

Combined functional genomic and chemical screens identify SETD8 as a therapeutic target in MYC-driven medulloblastoma.

Veo B1, Danis E1Pierce A1,2Sola I1Wang D1Foreman NK1,2,3Jin J4Ma A4Serkova N5Venkataraman S1,2Vibhakar R1,2,3,6.

 2018 May;24(5):572-579. doi: 10.1038/s41591-018-0006-x. Epub 2018 Apr 16. PMID 29662203

Potent antitumor efficacy of anti-GD2 CAR T cells in H3-K27M+ diffuse midline gliomas.

Mount CW1,2,3Majzner RG4Sundaresh S1Arnold EP1Kadapakkam M4Haile S4Labanieh L4,5Hulleman E6Woo PJ1Rietberg SP4Vogel H1,4,7,8Monje M9,10,11,12,13,14Mackall CL15,16,17.

 2018 May;20(5):432-442. doi: 10.1016/j.neo.2018.02.004. Epub 2018 Mar 22. PMID 29574250

A Pre-Clinical Assessment of the Pan-ERBB Inhibitor Dacomitinib in Pediatric and Adult Brain Tumors.


 2018 Jan;34(1):293-298. doi: 10.1002/btpr.2528. Epub 2017 Jul 31. PMID 28726331

Novel fluid shear-based dissociation device for improved single cell dissociation of spheroids and cell aggregates.


 2017 Sep 28;549(7673):533-537. doi: 10.1038/nature24014. Epub 2017 Sep 20. PMID 28959975 

Targeting neuronal activity-regulated neuroligin-3 dependency in high-grade glioma.


 2017 May 8;31(5):635-652.e6. doi: 10.1016/j.ccell.2017.03.011. Epub 2017 Apr 20. PMID 28434841

Transcriptional Dependencies in Diffuse Intrinsic Pontine Glioma.


 2017 Jan 19;24(1):87-97. doi: 10.1016/j.chembiol.2016.12.007. Epub 2017 Jan 5. PMID 28065656

APT2 Inhibition Restores Scribble Localization and S-Palmitoylation in Snail-Transformed Cells.


 2017 Apr 1;320:60-72. doi: 10.1016/j.taap.2017.02.012. Epub 2017 Feb 16. PMID 28213093

Enhanced effect of geldanamycin nanocomposite against breast cancer cells growing in vitro and as xenograft with vanquished normal cell toxicity.


 2017 Jun 20;(124). doi: 10.3791/55846. PMID 28654058

In Vitro Assays to Assess Blood-brain Barrier Mesh-like Vessel Formation and Disruption.


 2016 Nov;36(11):1865-1871. Epub 2016 Sep 15. PMID 27634936

Epidermal growth factor prevents oligomeric amyloid-β induced angiogenesis deficits in vitro.


 2016 May;12(6):1799-808. doi: 10.1039/c6mb00019c. PMID 27030425

Correlated S-palmitoylation profiling of Snail-induced epithelial to mesenchymal transition.


 2015 Nov;4(11):1265-74. doi: 10.5966/sctm.2015-0062. Epub 2015 Sep 10. PMID 26359426

Adult Lung Spheroid Cells Contain Progenitor Cells and Mediate Regeneration in Rodents With Bleomycin-Induced Pulmonary Fibrosis.


 2015 Mar 2;10(1):015026. doi: 10.1088/1748-6041/10/1/015026. PMID 25729882

Effects of epidermal growth factor-loaded mucoadhesive films on wounded oral tissue rafts.


 2015 Jan;23(1):99-107. doi: 10.1038/mt.2014.177. Epub 2014 Sep 9. PMID 25200130

Use of miRNA response sequences to block off-target replication and increase the safety of an unattenuated, glioblastoma-targeted oncolytic HSV.


 2015 Jun;21(6):555-9. doi: 10.1038/nm.3855. Epub 2015 May 4. PMID 25939062

Functionally defined therapeutic targets in diffuse intrinsic pontine glioma.


 2015 Jul 13;10(7):e0132823. doi: 10.1371/journal.pone.0132823. eCollection 2015. PMID 26167936

ROCK Inhibition Facilitates In Vitro Expansion of Glioblastoma Stem-Like Cells.


 2015 May;17(5):697-707. doi: 10.1093/neuonc/nou303. Epub 2014 Nov 2. PMID 25366336

Preclinical antitumor efficacy of selective exportin 1 inhibitors in glioblastoma.

 
 

 2014 Nov 27;33(48):5534-45. doi: 10.1038/onc.2013.499. Epub 2013 Nov 25. PMID 24276241 

cIAP1 regulates TNF-mediated cdc42 activation and filopodia formation.


 2014;6(9). pii: e207. doi: 10.7759/cureus.207. Epub 2014 Sep 17. PMID 28729960

A Bioengineered Peptide that Localizes to and Illuminates Medulloblastoma: A New Tool with Potential for Fluorescence-Guided Surgical Resection.


 2013 Sep 19;501(7467):380-4. doi: 10.1038/nature12530. Epub 2013 Sep 11. PMID 24025767
 2013 Mar;21(3):561-9. doi: 10.1038/mt.2012.211. Epub 2012 Oct 16. PMID 23070115

Effective treatment of an orthotopic xenograft model of human glioblastoma using an EGFR-retargeted oncolytic herpes simplex virus.

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