Human EBI3 subunit (IL-27/IL-35)

Epstein-Barr virus induced gene-3 (EBI3) is a secreted glycoprotein belonging to the hematopoietin receptor family related to the p40 subunit of interleukin 12 (IL-12).  EBI3 expression is induced in B-lymphocytes in response to Epstein-Barr virus infection.  EBI3 forms heterodimers with p28 to form interleukin 27 (IL-27), and with p35 to form interleukin 35 (IL-35).  Both IL-27 and IL-35 have anti-inflammatory and regulatory activity.     

SKU Select size Price Buy
100-162-5ug Mini: 5 ug $63.00 + -
100-162-20ug Std.: 20 ug $158.00 + -
100-162-100ug Value: 100 ug $609.00 + -
100-162-500ug Midgi: 500 ug $3,024.00 + -
100-162-1mg Mg: 1 mg $5,040.00 + -
100-162AF-5ug Animal-Free Mini: 5 ug $69.00 + -
100-162AF-20ug Animal-Free Std.: 20 ug $174.00 + -
100-162AF-100ug Animal-Free Value: 100 ug $667.00 + -
100-162AF-500ug Animal-Free Midgi: 500 ug $3,329.00 + -
100-162AF-1mg Animal-Free Mg: 1 mg $5,544.00 + -

Alternate Names:  IL-35/EBI3, IL-27/EBI3               Accession Number: Q14213
Description:  Epstein-Barr virus induced gene-3 (EBI3) is a secreted glycoprotein belonging to the hematopoietin receptor family related to the p40 subunit of interleukin 12 (IL-12).  EBI3 expression is induced in B-lymphocytes in response to Epstein-Barr virus infection.  EBI3 forms heterodimers with p28 to form interleukin 27 (IL-27), and with p35 to form interleukin 35 (IL-35).  Both IL-27 and IL-35 have anti-inflammatory and regulatory activity.     
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 23.4 kDa (210 aa)
AA Sequence:  MRKGPPAALT LPRVQCRASR YPIAVDCSWT LPPAPNSTSP VSFIATYRLG MAARGHSWPC LQQTPTSTSC TITDVQLFSM APYVLNVTAV HPWGSSSSFV PFITEHIIKP DPPEGVRLSP LAERQLQVQW EPPGSWPFPE IFSLKYWIRY KRQGAARFHR VGPIEATSFI LRAVRPRARY YVQVAAQDLT DYGELSDWSL PATATMSLGK
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) + 0.5% mannitol

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥90%

Endotoxin

Kinetic LAL

≤5 EUs/µg

Biological Activity

No biological activity data is available at this time

No biological activity data is available at this time 

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile 10 mM HCl at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or a bulk quote, email us at quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0615 Lot 0615
SDS Lot 0913 Lot 0913
PDS Animal-Free Lot 0615 Animal-Free  
SDS Animal-Free Lot 0913 Animal-Free  

 

 

Papers that cite this product:

 

Clin Immunology 2020 Aug;217:108498.  doi: 10.1016/j.clim.2020.108498. Epub 2020 Jun 10. PMID 32531345

IL-27: An endogenous constitutive repressor of human monocytes

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