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Human BDNF

Human BDNF, Brain-derived neurotrophic factor, is a recombinant protein used as a nerve growth factor. In tissue or cell culture, it supports neuron growth and survival. BDNF is often used in combination with recombinant human GDNF for neuronal stem cell cultures. Human BDNF, mouse BDNF, rat BDNF, and pig BDNF are cross-reactive.

Tested for biological activity in a C6 cell proliferation assay. Lyophilized protein, high purity, low endotoxin, high quality BDNF is manufactured in the USA by protein manufacturer Shenandoah.


SKU Select size Price Buy
100-01-2ug Mini: 2 ug $67.00 + -
100-01-10ug Std.: 10 ug $168.00 + -
100-01-100ug Value: 100 ug $646.00 + -
100-01-500ug Midgi: 500 ug $3,209.00 + -
100-01-1mg Mg: 1 mg $5,347.00 + -
100-01AF-2ug Animal-Free Mini: 2 ug $73.00 + -
100-01AF-10ug Animal-Free Std.: 10 ug $184.00 + -
100-01AF-100ug Animal-Free Value: 100 ug $713.00 + -
100-01AF-500ug Animal-Free Midgi: 500 ug $3,532.00 + -
100-01AF-1mg Animal-Free Mg: 1 mg $5,882.00 + -

Alternate Names:  None               Accession Number: P23560

Brain-derived neurotrophic factor (BDNF) is a nerve growth factor that binds two receptors, the low-affinity nerve growth factor receptor (LNGFR) and the tropomyosin receptor kinase B (TrkB), to support neuron growth and survival. BDNF expression in the hippocampus is essential for long-term memory storage and learning. Human, mouse, rat, and pig BDNF are cross-reactive.

Source:  Genetically modified E.coli.
Predicted MW: Noncovalent Homodimer, 13.6/27.3 kDa (120/240 aa)
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)

Product Specifications*

*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 


Method of Determination

Acceptance Criteria


Reducing and Non-Reducing SDS PAGE



Kinetic LAL

≤1 EUs/µg

Biological Activity

C6 cell proliferation

≤2 ug/mL; ≥ 5.0 x 10^2 units/mg

Preparation and Storage

Country of Origin:  USA   Shipping:  Room temperature             
Storage Prior to Reconstitution:  -20°C 
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date                   

  • 12 months from date of receipt when stored at -20°C to-80°C as supplied.
  • 1 month when stored at 4°C after reconstituting as directed.
  • 3 months when stored at -20°C to -80°C after reconstituting as directed.


Complementary products:
Need an Anti-Human BDNF antibody?  We recommend Anti-Human BDNF Rabbit Polyclonal Antibody from ImmunoReagents.  If you are curious to know more about the relationship between Shenandoah and Immunoreagents, click here for the scoop.


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Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 1209 Lot 0815
SDS Lot 0815  
PDS Animal-Free Lot 0410  
SDS Animal-Free  Lot 0815 Animal-Free  
Papers that cite this product:

 2019 Aug;95(8):825-835. doi: 10.1002/cyto.a.23783. Epub 2019 May 7. PMID 31063256

 2019 Jan;35(1):e2733. doi: 10.1002/btpr.2733. Epub 2018 Nov 17. PMID 30315732

Fabrication of thin-layer matrigel-based constructs for three-dimensional cell culture.

 2015 Feb 27;290(9):5311-27. doi: 10.1074/jbc.M114.602219. Epub 2015 Jan 5. PMID 25561733

The Na+/H+ exchanger NHE6 modulates endosomal pH to control processing of amyloid precursor protein in a cell culture model of Alzheimer disease.


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