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Mouse RANK Ligand
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Mouse RANK Ligand

Animal-Free (AF) proteins are identical to regular proteins and include additional documentation showing that they were produced with no materials of animal or human origin using ANIMAL-FREE purification processes.  Learn more HERE.

SKU Select size Price Buy
200-04-2ug Mini: 2 ug $63.00 + -
200-04-10ug Std.: 10 ug $158.00 + -
200-04-100ug Value: 100 ug $609.00 + -
200-04-500ug Midgi: 500 ug $3024.00 + -
200-04-1mg Mg: 1 mg $5040.00 + -
200-04AF-2ug Animal-Free Mini: 2 ug $69.00 + -
200-04AF-10ug Animal-Free Std.: 10 ug $174.00 + -
200-04AF-100ug Animal-Free Value: 100 ug $667.00 + -
200-04AF-500ug Animal-Free Midgi: 500 ug $3329.00 + -
200-04AF-1mg Animal-Free Mg: 1 mg $5544.00 + -
  • Weight
    0.0100 lbs
  • SKU
    200-04-2ug
Alternate Names:  TNFSF11, TRANCE, OPGL, ODF              
Accession Number: O35235
Description:  Receptor activator of nuclear factor kappa-B Ligand (RANK Ligand) is a cell-bound marker related to the tumor necrosis factor (TNF) family of proteins. RANK Ligand plays a critical role in bone metabolism and osteoclast differentiation. T cell expression of RANK Ligand promotes dendritic cell maturation.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 19.5 kDa (175 aa)
AA Sequence:  MPAMMEGSWLD VAQRGKPEAQ PFAHLTINAA SIPSGSHKVT LSSWYHDRGW AKISNMTLSN GKLRVNQDGF YYLYANICFR HHETSGSVPT DYLQLMVYVV KTSIKIPSSH NLMKGGSTKN WSGNSEFHFY SINVGGFFKL RAGEEISIQV SNPSLLDPDQ DATYFGAFKV QDID
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

Activity of RAW-BlueTM cells 

ED50 ≤ 50 ng/mL (or ≥ 2.0 x 10^4 units/mg)

In addition to the acceptance criteria, this product has been tested with the following assays:
(1) TNF-alpha production in RAW cells (typical ED50 is 50-75 ng/mL)
(2) Osteoclast formation (first detectable at 50 ng/mL)

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

 

Complementary antibody products:
Need an Anti-Mouse RANK-Ligand antibody?  We recommend:
Anti-Mouse RANK-Ligand Polyclonal Antibody from ImmunoReagents.  If you are curious to know more about the relationship between Shenandoah and Immunoreagents, click here for the scoop.

To receive more information or a quote, complete our bulk/custom order form.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0415 Lot 0415
SDS Lot 0415 Animal-Free  
PDS Animal-Free    
SDS Animal-Free    

 

 

Papers that use this product:
 
 
 
 2015 May;51:313-319. doi: 10.1016/j.biomaterials.2015.01.076. Epub 2015 Mar 4.

Tissue engineering a surrogate niche for metastatic cancer cells.


 
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