Mouse M-CSF Gel
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Mouse M-CSF

Animal-Free (AF) proteins are identical to regular proteins and include additional documentation showing that they were produced with no materials of animal or human origin using ANIMAL-FREE purification processes.  Learn more HERE.

SKU Select size Price Buy
200-08-2ug Mini: 2 ug $63.00 + -
200-08-10ug Std.: 10 ug $158.00 + -
200-08-100ug Value: 100 ug $510.00 + -
200-08-500ug Midgi: 500 ug $2457.00 + -
200-08-1mg Mg: 1 mg $4095.00 + -
200-08AF-2ug Animal-Free Mini: 2 ug $69.00 + -
200-08AF-10ug Animal-Free Std.: 10 ug $174.00 + -
200-08AF-100ug Animal-Free Value: 100 ug $562.00 + -
200-08AF-500ug Animal-Free Midgi: 500 ug $2704.00 + -
200-08AF-1mg Animal-Free Mg: 1 mg $4505.00 + -
  • Weight
    0.0100 lbs
  • SKU
    200-08-2ug

Alternate Names:  MGI-IM, CSF-1              

Accession Number: P07141
Description:  Macrophage colony stimulating factor (M-CSF) is a hematopoietic growth factor that is widely produced by a variety of cells.  M-CSF stimulates the proliferation and differentiation of hematopoietic stem cells into monocyte and macrophage cell types.  M-CSF also acts through the colony stimulating factor 1 receptor (CSF1R) to modulate processes involved in immunology, bone metabolism, fertility, and pregnancy.  Human M-CSF shows activity on mouse cells; however, mouse M-CSF shows no activity on human cells.  
Source:  Genetically modified E.coli.
Predicted MW: Dimer, 18.2/36.4 kDa (156/312 aa)
AA Sequence:  MKEVSEHCSH MIGNGHLKVL QQLIDSQMET SCQIAFEFVD QEQLDDPVCY LKKAFFLVQD IIDETMRFKD NTPNANATER LQELSNNLNS CFTKDYEEQN KACVRTFHET PLQLLEKIKN FFNETKNLLE KDWNIFTKNC NNSFAKCSSR DVVTKP
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

NFS-60 cell proliferation

≤10 ng/mL; ≥ 1.0 x 10^5 units/mg

 

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

 

To receive more information or a quote, complete our bulk/custom order form.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 1213 Lot 1213
SDS Lot 0507  
PDS Animal-Free Lot 1213 Animal-Free   
SDS Animal-Free    

 

 

 

Papers that use this product:
 
 
 
 2019 Aug 27;7(3). pii: E101. doi: 10.3390/vaccines7030101.


 2015 Feb 15;331(2):320-30. doi: 10.1016/j.yexcr.2014.10.015. Epub 2014 Oct 24.

Macrophages enhance tumor-derived autophagosomes (DRibbles)-induced B cells activation by TLR4/MyD88 and CD40/CD40L.

Zhou M1Li W1Wen Z1Sheng Y1Ren H2Dong H1Cao M1Hu HM3Wang LX4.

 2014 Oct;147(4):835-46. doi: 10.1053/j.gastro.2014.06.024. Epub 2014 Jun 21.
 2013 Jan 15;73(2):662-71. doi: 10.1158/0008-5472.CAN-12-0653.

Twist1 induces CCL2 and recruits macrophages to promote angiogenesis.


 2013 Dec 4;8(12):e81269. doi: 10.1371/journal.pone.0081269. eCollection 2013.

Lack of RNase L attenuates macrophage functions.


 2008 Sep 30;338(1-2):47-57. doi: 10.1016/j.jim.2008.07.009. Epub 2008 Aug 26.

An efficient culture method for generating large quantities of mature mouse splenic macrophages.

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