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Mouse CD40-Ligand
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Mouse CD40-Ligand

TNFSF5, TRAP, CD154, gp39, T-BAM 

SKU Select size Price Buy
200-10-5ug Mini: 5 ug $63.00 + -
200-10-25ug Std.: 25 ug $158.00 + -
200-10-100ug Value: 100 ug $394.00 + -
200-10-500ug Midgi: 500 ug $1701.00 + -
200-10-1mg Mg: 1 mg $2835.00 + -
200-10AF-5ug Animal-Free Mini: 5 ug $69.00 + -
200-10AF-25ug Animal-Free Std.: 25 ug $174.00 + -
200-10AF-100ug Animal-Free Value: 100 ug $436.00 + -
200-10AF-500ug Animal-Free Midgi: 500 ug $1869.00 + -
200-10AF-1mg Animal-Free Mg: 1 mg $3119.00 + -
  • Weight
    0.0100 lbs
  • SKU
    200-10-5ug

Alternate Names:  TNFSF5, TRAP, CD154, gp39, T-BAM               Accession Number: P27548
Description:  CD40-Ligand, or CD154, is a membrane glycoprotein and differentiation antigen that is expressed on the surface of T cells, monocytes, basophils, eosinophils, platelets, dendritic cells, and endothelial cells. CD40-Ligand binds and activates the CD40 receptor on antigen-presenting cells. CD40-Ligand stimulates B cell proliferation, immunoglobulin class switching, and antibody secretion. CD40-Ligand also induces cytokine production and tumoricidal activity in peripheral blood monocytes. CD40-Ligand is a co-stimulator of activated T cell proliferation and inflammatory protein production.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 16.4 kDa (149 aa)
AA Sequence:  MQRGDEDPQI AAHVVSEANS NAASVLQWAK KGYYTMKSNL VMLENGKQLT VKREGLYYVY TQVTFCSNRE PSSQRPFIVG LWLKPSSGSE RILLKAANTH SSSQLCEQQS VHLGGVFELQ AGASVFVNVT EASQVIHRVG FSSFGLLKL
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM arginine, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

Activity of HEK-BlueTM CD40L cells

ED50 ≤ 50 ng/mL; ≥ 2.0 x 10^4 units/mg

 

In addition to the acceptance criteria, this product has been tested with the following assay:
(1) Induced IL-8 production in human PBMCs detected via ELISA (typical ED50 is 1-10 ng/mL)

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date                      
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or a quote, complete our bulk/custom order form.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 08182 Lot 08181
SDS Lot 08181 Lot 08182
PDS Animal-Free Lot 0213   
SDS Animal-Free Lot 0113   
  Lot 08181 Animal-Free  
  Lot 08182 Animal-Free  

 

 

 

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    • $63.00