Human VEGF-165

Vascular endothelial growth factor-A (VEGF-A) is produced by a wide variety of cell types, including tumor and vascular cells. VEGF-A is a mediator of vascular growth, vascular permeability, and plays a role in stimulating vasodilation via nitric oxide-dependent pathways.

SKU Select size Price Buy
100-44-2ug Mini: 2 ug $63.00 + -
100-44-10ug Std.: 10 ug $158.00 + -
100-44-100ug Value: 100 ug $457.00 + -
100-44-500ug Midgi: 500 ug $2,268.00 + -
100-44-1mg Mg: 1 mg $3,780.00 + -
100-44AF-2ug Animal-Free Mini: 2 ug $69.00 + -
100-44AF-10ug Animal-Free Std.: 10 ug $174.00 + -
100-44AF-100ug Animal-Free Value: 100 ug $499.00 + -
100-44AF-500ug Animal-Free Midgi: 500 ug $2,494.00 + -
100-44AF-1mg Animal-Free Mg: 1 mg $4,158.00 + -
Alternate Names:  VEGF-A, VPF, glioma-derived endothelial cell mitogen              
Accession Number: P15692-4
Description:  Vascular endothelial growth factor-A (VEGF-A) is produced by a wide variety of cell types, including tumor and vascular cells. VEGF-A is a mediator of vascular growth, vascular permeability, and plays a role in stimulating vasodilation via nitric oxide-dependent pathways. VEGF-A has several alternatively spliced isoforms, with VEGF-165 being the most abundant. The VEGF-165 isoform is a secreted protein that acts on receptors VEGFR-1 and VEGFR-2 to modulate endothelial cell proliferation and angiogenesis.
Source:  Genetically modified E.coli.
Predicted MW: Dimer, 19.3/38.6 kDa (166/332 aa)
AA Sequence:  MAPMAEGGGQ NHHEVVKFMD VYQRSYCHPI ETLVDIFQEY PDEIEYIFKP SCVPLMRCGG CCNDEGLECV PTEESNITMQ IMRIKPHQGQ HIGEMSFLQH NKCECRPKKD RARQENPCGP CSERRKHLFV QDPQTCKCSC KNTDSRCKAR QLELNERTCR CDKPRR
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1%
 
Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

HUVEC proliferation

≤10 ng/mL

Preparation and Storage

Country of Origin:  USA   

Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C

Product Reconstitution:  Sterile water at 0.1 mg/mL

Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date        

  • 12 months from date of receipt when stored at -20°C to-80°C as supplied.
  • 1 month when stored at 4°C after reconstituting as directed.
  • 3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 1009 Lot 0118
SDS Lot 1007 Lot 0914
PDS Animal-Free Lot 0914  
SDS Animal-Free Lot 0113  
  Lot 0914 Animal-Free  
  Lot 0113 Animal-Free  
Papers that use this product:
 
 
bioRxiv  Apr 2020 

Desert Hedgehog-driven endothelium integrity is enhanced by Gas1 but negatively regulated by Cdon

Candice ChapoulyPierre-Louis HollierSarah GuimbalLauriane CornuaultAlain-Pierre GadeauMarie-Ange Renault 
 2019 Jun 18;91(12):7603-7612. doi: 10.1021/acs.analchem.9b00238. Epub 2019 May 30. PMID 31088078
 2018 Dec 5;7:103. doi: 10.1038/s41377-018-0093-y. eCollection 2018. PMID 30534372

High-resolution, in vivo multimodal photoacoustic microscopy, optical coherence tomography, and fluorescence microscopy imaging of rabbit retinal neovascularization.

Zhang W1,2Li Y3,4Nguyen VP3Huang Z1Liu Z2Wang X1,5Paulus YM1,3.

 2016 Nov 14;17(11):3724-3731. Epub 2016 Nov 1. PMID 27744681

Porous Silicon-Based Cell Microarrays: Optimizing Human Endothelial Cell-Material Surface Interactions and Bioactive Release.


 2016 Jan 13;8(1):802-8. doi: 10.1021/acsami.5b10036. Epub 2015 Dec 28. PMID 26710239

Mutually-Reactive, Fluorogenic Hydrocyanine/Quinone Reporter Pairs for In-Solution Biosensing via Nanodroplet Association.


 2015 Feb;41(2):277-82. doi: 10.1111/jog.12511. Epub 2014 Sep 26. PMID 25255906

Vascular endothelial growth factor, soluble fms-like tyrosine kinase 1 and genistein-induced changes in the vascular reactivity of rat's aorta.

 2014 Sep;24(3):216-22. doi: 10.3109/08982104.2014.891230. Epub 2014 Mar 6. PMID 24597467

Use of thermodynamic coupling between antibody-antigen binding and phospholipid acyl chain phase transition energetics to predict immunoliposome targeting affinity.


 2014 Apr;35(11):3678-87. doi: 10.1016/j.biomaterials.2013.12.065. Epub 2014 Jan 27. PMID 24480538

Characteristics of platelet gels combined with silk.


 2014 Apr;3(2):225-44. doi: 10.1002/cam4.188. Epub 2014 Jan 22. PMID 24449499

Quantitative fluorescent profiling of VEGFRs reveals tumor cell and endothelial cell heterogeneity in breast cancer xenografts.


 2014 Jan;35(1):83-91. doi: 10.1016/j.biomaterials.2013.09.053. Epub 2013 Oct 4. PMID 24099708

Multifunctional silk-heparin biomaterials for vascular tissue engineering applications.


 2014 Sep;20(17-18):2503-12. doi: 10.1089/ten.TEA.2013.0249. Epub 2014 May 9. PMID 24702044

Synergism of matrix stiffness and vascular endothelial growth factor on mesenchymal stem cells for vascular endothelial regeneration.


 2012 Apr;23(7):1388-98. doi: 10.1091/mbc.E11-09-0811. Epub 2012 Feb 9. PMID 22323292

Nitric oxide-dependent Src activation and resultant caveolin-1 phosphorylation promote eNOS/caveolin-1 binding and eNOS inhibition.


 2011 Jan 7;286(1):737-45. doi: 10.1074/jbc.M110.177006. Epub 2010 Oct 25. PMID 20974861

Heparan sulfate regulates VEGF165- and VEGF121-mediated vascular hyperpermeability.


 2011 Apr 15;317(7):955-65. doi: 10.1016/j.yexcr.2010.12.014. Epub 2010 Dec 23. PMID 21185287

Quantification and cell-to-cell variation of vascular endothelial growth factor receptors.


 2011 Jan 15;26(5):2442-7. doi: 10.1016/j.bios.2010.10.029. Epub 2010 Oct 23. PMID 21081271

A folding-based electrochemical aptasensor for detection of vascular endothelial growth factor in human whole blood.


 2009 Sep;30(27):4618-28. doi: 10.1016/j.biomaterials.2009.05.030. Epub 2009 Jun 21. PMID 19540581

The phosphorylation of vascular endothelial growth factor receptor-2 (VEGFR-2) by engineered surfaces with electrostatically or covalently immobilized VEGF.


 2008 Sep;173(3):689-99. doi: 10.2353/ajpath.2008.080088. Epub 2008 Aug 7. PMID 18688025 

Epidermal vascular endothelial growth factor production is required for permeability barrier homeostasis, dermal angiogenesis, and the development of epidermal hyperplasia: implications for the pathogenesis of psoriasis.

 

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