Human TNF-alpha

Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever. 

SKU Select size Price Buy
100-111-10ug Mini: 10 ug $63.00 + -
100-111-50ug Std.: 50 ug $158.00 + -
100-111-100ug Value: 100 ug $284.00 + -
100-111-500ug Midgi: 500 ug $725.00 + -
100-111-1mg Mg: 1 mg $1,260.00 + -
100-111AF-10ug Animal-Free Mini: 10 ug $69.00 + -
100-111AF-50ug Animal-Free Std.: 50 ug $174.00 + -
100-111AF-100ug Animal-Free Value: 100 ug $310.00 + -
100-111AF-500ug Animal-Free Midgi: 500 ug $798.00 + -
100-111AF-1mg Animal-Free Mg: 1 mg $1,386.00 + -

Alternate Names:  TNFSF2, Cachectin, DIF, Necrosin, Cytotoxin, Cachexin, TNF               Accession Number: P01375
Description:  Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever. 

Source:  Genetically modified E.coli.
Predicted MW: Monomer, 17.5 kDa (158 aa)
AA Sequence:  MVRSSSRTPS DKPVAHVVAN PQAEGQLQWL NRRANALLAN GVELRDNQLV VPSEGLYLIY SQVLFKGQGC PSTHVLLTHT ISRIAVSYQT KVNLLSAIKS PCQRETPEGA EAKPWYEPIY LGGVFQLEKG DRLSAEINRP DYLDFAESGQ VYFGIIAL
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

Cytolysis of mouse L929 cells in the presence of Actinomycin D

≤2 ng/mL; ≥ 5.0 x 10^5 units/mg 

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature              Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening.  Suspend the product by gently pipetting the above recommended solution down the sides of the vial.  DO NOT VORTEX.  Allow several minutes for complete reconstitution.  For prolonged storage, dilute to working aliquots in a 0.1% BSA solution.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.

 

To receive more information or for a bulk quote, email quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0411 Lot 0313
SDS Lot 0313  
PDS Animal-Free Lot 0213  
SDS Animal-Free  Lot 0313 Animal-Free  
Papers that use this product:
 
 
 

 2019 Aug 1;11(8). pii: E450. doi: 10.3390/toxins11080450. PMID 31374990
 2018 Aug;25(8):687-697. doi: 10.1038/s41594-018-0102-0. Epub 2018 Aug 3. PMID 30076409

RNAs interact with BRD4 to promote enhanced chromatin engagement and transcription activation.


 2018 Nov 1;189:585-591. doi: 10.1016/j.talanta.2018.07.055. Epub 2018 Jul 18. PMID 30086964

Effects of redox label location on the performance of an electrochemical aptamer-based tumor necrosis factor-alpha sensor.


 2018 Aug 24;293(34):13234-13246. doi: 10.1074/jbc.RA118.003387. Epub 2018 Jun 28. PMID 29954944

Mutant p53 regulates enhancer-associated H3K4 monomethylation through interactions with the methyltransferase MLL4.

 

 

 2016 Aug 4;329:30-42. doi: 10.1016/j.neuroscience.2016.04.033. Epub 2016 Apr 27. PMID 27132231

Inhibition of cerebral vascular inflammation by brain endothelium-targeted oligodeoxynucleotide complex.



 2016 Feb 1;196(3):1070-80. doi: 10.4049/jimmunol.1501310. Epub 2015 Dec 28. PMID 26712946 

The Notch Ligand DLL4 Defines a Capability of Human Dendritic Cells in Regulating Th1 and Th17 Differentiation.


 2014 Nov 27;33(48):5534-45. doi: 10.1038/onc.2013.499. Epub 2013 Nov 25. PMID 24276241 

cIAP1 regulates TNF-mediated cdc42 activation and filopodia formation.


 2014 Apr;99(4):393-406. doi: 10.1007/s12185-014-1534-x. Epub 2014 Feb 22. PMID 24563081

Nov/CCN3 regulates long-term repopulating activity of murine hematopoietic stem cells via integrin αvβ3.


  2012 May 4;418(3-4):134-44. doi: 10.1016/j.jmb.2011.12.053. Epub 2011 Dec 29. PMID 22227388

Engineering and structural characterization of a linear polyubiquitin-specific antibody.

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