Human TGF-beta 1 Gel
Zoom image

Human TGF beta 1

Differentiation inhibiting factor, cartilage-inducing factor 

SKU Select size Price Buy
100-39-5ug Mini: 5 ug $79.00 + -
100-39-20ug Std: 20 ug $284.00 + -
100-39-100ug Value: 100 ug $1045.00 + -
100-39-500ug Midgi: 500 ug $3145.00 + -
100-39-1mg Mg: 1 mg $5245.00 + -
  • Weight
    0.0100 lbs
  • SKU
    100-39-5ug

Alternate Names:  Differentiation inhibiting factor, cartilage-inducing factor               Accession Number: P01137
Description:  Transforming growth factor-beta 1 (TGF-b1) is a member of the TGF-beta superfamily of cytokines. Members of this family exhibit regulatory activity in immunity, proliferation, adhesion, migration, and growth inhibition pathways. TGF-b1 signals through SMAD proteins via the TGF-bRI and TGF-bRII receptors.  TGF-b1 is often used in addition to recombinant IL-2 for production of Treg cells in cell culture.  
Source:  Genetically modified CHO cells.
Predicted MW: Dimer, 12.8/25.6 kDa (112/224 aa)
AA Sequence:  ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1 % trifluoroacetic acid (TFA) and Trehalose in a 20:1 Trehalose to protein ratio

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

Inhibition of IL-4-induced HT-2 cell proliferation

≤500 pg/mL; ≥ 2.0 x 10^6 units/mg

 

 **** Reference Units: The activity of Human TFG-beta is 2.2 x 10^4 RU/μg, which was calibrated against recombinant human TGF-beta 1 NIBSC code: 89/514.

Preparation and Storage

Country of Origin:  USA       

Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C

Product Reconstitution:  Sterile 10 mM HCl at 0.1 mg/mL

Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:      

  • 12 months from date of receipt when stored at -20°C to -80°C as supplied.
  • 1 month when stored at 4°C after reconstituting as directed.
  • 3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or a quote, complete our bulk/custom order form.
Papers that use this product:
 
 
 2019 Feb;28(2):161-168. doi: 10.1111/exd.13864.

Tissue microenvironment initiates an immune response to structural components of Staphylococcus aureus.


Biol Chem 2018 Feb 23;399(3):253-264.  doi: 10.1515/hsz-2017-0217

TGF-β Requires the Activation of Canonical and Non-Canonical Signalling Pathways to Induce Skeletal Muscle Atrophy


 2017 Jun;66(6):737-751. doi: 10.1007/s00262-017-1965-3. Epub 2017 Mar 9.

Murine Th17 cells utilize IL-2 receptor gamma chain cytokines but are resistant to cytokine withdrawal-induced apoptosis.


 2016;40(1-2):27-38. Epub 2016 Nov 14.

Angiotensin-(1-7) Prevents Skeletal Muscle Atrophy Induced by Transforming Growth Factor Type Beta (TGF-β) via Mas Receptor Activation.


 2015 Oct;17(5):376-92. doi: 10.1089/cell.2015.0040. Epub 2015 Aug 28.

Characterization and Multilineage Differentiation of Domestic and Black-Footed Cat Mesenchymal Stromal/Stem Cells from Abdominal and Subcutaneous Adipose Tissue.



 2015 Sep 15;22(10):885-93. doi: 10.1016/j.phymed.2015.06.011. Epub 2015 Jul 8.

Apocynin inhibits the upregulation of TGF-β1 expression and ROS production induced by TGF-β in skeletal muscle cells.



 2014 Oct 1;549(1):134-40. doi: 10.1016/j.gene.2014.07.056. Epub 2014 Jul 23.

A role for IL-10 in the transcriptional regulation of Roquin-1.

 

 2014 Oct 23;28(16):2375-80. doi: 10.1097/QAD.0000000000000431.

Transforming growth factor beta 1 up-regulates CD169 (sialoadhesin) expression on monocyte-derived dendritic cells: role in HIV sexual transmission.


 2012 Dec 15;303(12):G1402-15. doi: 10.1152/ajpgi.00071.2012. Epub 2012 Oct 11.

Hepatic microtubule acetylation and stability induced by chronic alcohol exposure impair nuclear translocation of STAT3 and STAT5B, but not Smad2/3.

 
 
 
 
Related products
Search Tip: To help narrow down your search results try:

  • Search product name, no species.
  • Select 'Exact phrase' match
If you're unable to find what you are looking for please contact us.
Drop items here to shop
Product has been added to your cart
  • Drag and drop me to the cart
    • $63.00
  • Drag and drop me to the cart
    • $378.00
  • Drag and drop me to the cart
    • $189.00
  • Drag and drop me to the cart
    • $798.00
  • Drag and drop me to the cart
    • $924.00
  • Drag and drop me to the cart
    • $189.00