Human PDGF-AB

Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells.

SKU Select size Price Buy
100-17-2ug Mini: 2 ug $63.00 + -
100-17-10ug Std.: 10 ug $158.00 + -
100-17-100ug Value: 100 ug $447.00 + -
100-17-500ug Midgi: 500 ug $2,205.00 + -
100-17-1mg Mg: 1 mg $3,675.00 + -
100-17AF-2ug Animal-Free Mini: 2 ug $69.00 + -
100-17AF-10ug Animal-Free Std.: 10 ug $174.00 + -
100-17AF-100ug Animal-Free Value: 100 ug $489.00 + -
100-17AF-500ug Animal-Free Midgi: 500 ug $2,426.00 + -
100-17AF-1mg Animal-Free Mg: 1 mg $4,043.00 + -

Alternate Names:  GDGF, ODGF               Accession Number: P04085/P01127
Description:  Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis. PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β and EGF signaling. PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells. PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues. PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB). The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β.
Source:  Genetically modified E.coli.
Predicted MW: Dimer, Alpha:14.4 kDa, Beta: 12.4 kDa, Total 26.8 kDa (Alpha: 126, Beta: 110, Total: 236 aa)
AA Sequence:  Alpha chain: MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT Beta chain: MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETVAAARPVT
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

3T3 cell proliferation

≤20 ng/mL; ≥ 5.0 x 10^4 units/mg

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or a bulk quote, email us at quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0609 Lot 0613
SDS Lot 0613  
PDS Animal-Free Lot 0613 Animal-Free  
SDS Animal-Free    
Papers that use this product:
 
 
 2019 Mar 4;10(1):1023. doi: 10.1038/s41467-019-08823-9. PMID 30833574

ACVR1 R206H cooperates with H3.1K27M in promoting diffuse intrinsic pontine glioma pathogenesis.


 2017 Nov 24;8(1):1780. doi: 10.1038/s41467-017-01955-w. PMID 29176654

Programmed biomolecule delivery to enable and direct cell migration for connective tissue repair.

Qu F1,2,3Holloway JL2,3Esterhai JL2Burdick JA4,5Mauck RL6,7,8.

 2017 May 8;31(5):635-652.e6. doi: 10.1016/j.ccell.2017.03.011. Epub 2017 Apr 20. PMID 28434841

Transcriptional Dependencies in Diffuse Intrinsic Pontine Glioma.


 2015 May;17(5):697-707. doi: 10.1093/neuonc/nou303. Epub 2014 Nov 2. PMID 25366336

Preclinical antitumor efficacy of selective exportin 1 inhibitors in glioblastoma.

 

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