Human PDGF-AA

Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis.  PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β and EGF signaling.  PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells.  PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues.  PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB).  The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β. 

SKU Select size Price Buy
100-16-2ug Mini: 2 ug $63.00 + -
100-16-10ug Std.: 10 ug $158.00 + -
100-16-100ug Value: 100 ug $447.00 + -
100-16-500ug Midgi: 500 ug $2,205.00 + -
100-16-1mg Mg: 1 mg $3,675.00 + -
100-16AF-2ug Animal-Free Mini: 2 ug $69.00 + -
100-16AF-10ug Animal-Free Std.: 10 ug $174.00 + -
100-16AF-100ug Animal-Free Value: 100 ug $489.00 + -
100-16AF-500ug Animal-Free Midgi: 500 ug $2,426.00 + -
100-16AF-1mg Animal-Free Mg: 1 mg $4,043.00 + -

Alternate Names:  GDGF, ODGF               Accession Number: P04085
Description:  Platelet-derived growth factor (PDGF) is an important regulator of cell growth, proliferation, and angiogenesis.  PDGF synthesis is induced by IL-1, IL-6, TNF-α, TGF-β and EGF signaling.  PDGF functions as a mitogenic growth hormone on cells of mesenchymal lineage, such as smooth muscle and glial cells.  PDGF is also stored in the alpha-granules of platelets and is released upon adherence to traumatized tissues.  PDGF is a dimeric glycoprotein formed by two A chains (AA), two B chains (BB), or as a heterodimer with an A and a B chain (AB).  The PDGF dimer binds the cell surface receptor tyrosine kinases PDGFR-α and PDGFR-β. 
Source:  Genetically modified E.coli.
Predicted MW: Dimer, 14.4/28.9 kDa (126/252 aa)
AA Sequence:  MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

3T3 cell proliferation

≤50 ng/mL; ≥ 2.0 x 10^4 units/mg

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or a bulk quote, email us at quotes@shenandoah-bt.com.

 

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 1011 Lot 0613
SDS Lot 0613  
PDS Animal-Free Lot 0613 Animal-Free   
SDS Animal-Free    
Papers that use this product:
 
 
July 2020 UC Santa Cruz. ProQuest ID: Sanders_ucsc_0036E_12024. Merritt ID: ark:/13030/m5102czj. Retrieved from https://escholarship.org/uc/item/8452n2gj
 
 
Sanders, L. M. (2020)
 

Neuron 2019 Jul 17;103(2):250-265.e8.  doi: 10.1016/j.neuron.2019.04.032. Epub 2019 May 20. PMID 31122677

Loss of Adaptive Myelination Contributes to Methotrexate Chemotherapy-Related Cognitive Impairment

 2019 May 9;2:156. doi: 10.1038/s42003-019-0420-8. eCollection 2019. PMID 31098401
 2018 May;24(5):572-579. doi: 10.1038/s41591-018-0006-x. Epub 2018 Apr 16. PMID 29662203

Potent antitumor efficacy of anti-GD2 CAR T cells in H3-K27M+ diffuse midline gliomas.

Mount CW1,2,3Majzner RG4Sundaresh S1Arnold EP1Kadapakkam M4Haile S4Labanieh L4,5Hulleman E6Woo PJ1Rietberg SP4Vogel H1,4,7,8Monje M9,10,11,12,13,14Mackall CL15,16,17.
 2017 Feb 2;20(2):233-246.e7. doi: 10.1016/j.stem.2016.11.003. Epub 2016 Dec 15. PMID 27989769

Adaptive Chromatin Remodeling Drives Glioblastoma Stem Cell Plasticity and Drug Tolerance.


 2017 Sep 28;549(7673):533-537. doi: 10.1038/nature24014. Epub 2017 Sep 20. PMID 28959975

Targeting neuronal activity-regulated neuroligin-3 dependency in high-grade glioma.

 

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