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Human Myostatin

Myostatin, also known as GDF-8, is a conserved member of the TGF-beta superfamily. Myostatin is an essential regulator of skeletal muscle mass and cardiac muscle development and function. Myostatin is a secreted protein that negatively regulates skeletal muscle growth by determining muscle fiber number and size. Myostatin binds one of the two activin type II receptors (ACTRIIA or ACTRIIB) to activate SMAD signaling.

SKU Select size Price Buy
100-22-2ug Mini: 2 ug $65.00 + -
100-22-10ug Std.: 10 ug $163.00 + -
100-22-100ug Value: 100 ug $627.00 + -
100-22-500ug Midgi: 500 ug $3,115.00 + -
100-22-1mg Mg: 1 mg $5,191.00 + -
100-22AF-2ug Animal-Free Mini: 2 ug $70.00 + -
100-22AF-10ug Animal-Free Std.: 10 ug $178.00 + -
100-22AF-100ug Animal-Free Value: 100 ug $687.00 + -
100-22AF-500ug Animal-Free Midgi: 500 ug $3,429.00 + -
100-22AF-1mg Animal-Free Mg: 1 mg $5,710.00 + -
Alternate Names:  GDF-8, MSTN               Accession Number: O14793(267-375)
Description:  Myostatin, also known as GDF-8, is a conserved member of the TGF-beta superfamily. Myostatin is an essential regulator of skeletal muscle mass and cardiac muscle development and function. Myostatin is a secreted protein that negatively regulates skeletal muscle growth by determining muscle fiber number and size. Myostatin binds one of the two activin type II receptors (ACTRIIA or ACTRIIB) to activate SMAD signaling. Myostatin also activates MAPK signaling through TAK1-MKK6 and Ras pathways. Inhibition of myostatin increases muscle mass in a number of human disease animal models, such as muscular dystrophy.
Source:  Genetically modified E.coli.
Predicted MW: Dimer, 12.4/24.8 kDa (109/218 aa)
AA Sequence:  DFGLDCDEHS TESRCCRYPL TVDFEAFGWD WIIAPKRYKA NYCSGECEFV FLQKYPHTHL VHQANPRGSA GPCCTPTKMS PINMLYFNGK EQIIYGKIPA MVVDRCGCS
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

MPC-11 cell cytotoxicity

≤50 ng/ml; ≥ 2.0 x 10^4 units/mg

Preparation and Storage

Country of Origin:  USA        Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile 20 mM HCl at 0.1 mg/mL

Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0909 Lot 0817
SDS Lot 0112  
PDS Animal-Free Lot 0817  
SDS Animal-Free Lot 0817 Animal-Free  

 

 

Papers that use this product:

 

 2013 May;20(5):522-9. doi: 10.1111/j.1442-2042.2012.03186.x. Epub 2012 Oct 10. PMID 23050675

Myostatin inhibits proliferation of human urethral rhabdosphincter satellite cells.


Growth Factors 2009 Aug;27(4):247-54.  doi: 10.1080/08977190903052539. PMID 19591015

Myostatin in Tendon Maintenance and Repair

 

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