Human M-CSF

Human MCSF, Macrophage Colony Stimulating Factor, is a recombinant protein used in cell culture to differentiate primary PBMCs & monocytes to macrophages. Tested for biological activity in M-NFS-60 cell proliferation assay. Lyophilized protein, high purity, low endotoxin & high quality M-CSF manufactured in the USA by protein manufacturer Shenandoah.

SKU Select size Price Buy
100-03-2ug Mini: 2 ug $63.00 + -
100-03-10ug Std.: 10 ug $158.00 + -
100-03-100ug Value: 100 ug $510.00 + -
100-03-500ug Midgi: 500 ug $2,457.00 + -
100-03-1mg Mg: 1 mg $4,095.00 + -
100-03AF-2ug Animal-Free Mini: 2 ug $69.00 + -
100-03AF-10ug Animal-Free Std.: 10 ug $174.00 + -
100-03AF-100ug Animal-Free Value: 100 ug $562.00 + -
100-03AF-500ug Animal-Free Midgi: 500 ug $2,704.00 + -
100-03AF-1mg Animal-Free Mg: 1 mg $4,505.00 + -

Macrophage colony stimulating factor (M-CSF) is a hematopoietic growth factor that is widely produced by a variety of cells. M-CSF stimulates the proliferation and differentiation of hematopoietic stem cells into monocyte and macrophage cell types. M-CSF also acts through the colony stimulating factor 1 receptor (CSF1R) to modulate processes involved in immunology, bone metabolism, fertility, and pregnancy. Human M-CSF shows activity on mouse cells; however, mouse M-CSF shows no activity on human cells.
Source:  Genetically modified E.coli.

Catalog Number: 100-03

Alternate Names:  MGI-IM, CSF-1               Accession Number: P09603

Predicted MW: Dimer, 18.5/37.1 kDa (159/318 aa)
AA Sequence:  MEEVSEYCSH MIGSGHLQSL QRLIDSQMET SCQITFEFVD QEQLKDPVCY LKKAFLLVQD IMEDTMRFRD NTPNAIAIVQ LQELSLRLKS CFTKDYEEHD KACVRTFYET PLQLLEKVKN VFNETKNLLD KDWNIFSKNC NNSFAECSSQ GHERQSEGS
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 100 mM sodium chloride, pH 8.0

M-CSF Product Specifications*

*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

NFS-60 cell proliferation

≤10 ng/mL; ≥ 1.0 x 10^5 units/mg

 

 *** International Units: The specific activity of Human M-CSF is 1.0 x 10^5
 IU/μg, which is calibrated against recombinant human M-CSF WHO International Standard (NIBSC code: 89/512).

Preparation and Storage

Country of Origin:  USA       

Shipping:  Room temperature             Storage Prior to Reconstitution:  -20°C

Product Reconstitution:  Sterile water at 0.1 mg/mL

Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date

12 months from date of receipt when stored at -20°C to-80°C as supplied.

1 month when stored at 4°C after reconstituting as directed.

3 months when stored at -20°C to -80°C after reconstituting as directed.

 

To receive more information or for a bulk quote, email quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0311 Lot 0114
SDS Lot 1008 Lot 1117
PDS Animal-Free Lot 1117  
SDS Animal-Free Lot 1117 Animal-Free  
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