Human IL-4

Interleukin 4 (IL-4) is an immunomodulatory cytokine that functions to induce naïve helper T cells to differentiate into type 2 T helper (Th2) cells. Th2 cells subsequently produce more IL-4 in a positive feedback loop. IL-4 also promotes immunoglobulin IgG to IgE isotype switching on B cells. IL-4 binds the IL-4Rα receptor to activate STAT6 signaling.

SKU Select size Price Buy
100-09-5ug Mini: 5 ug $63.00 + -
100-09-20ug Std.: 20 ug $158.00 + -
100-09-100ug Value: 100 ug $410.00 + -
100-09-500ug Midgi: 500 ug $2,016.00 + -
100-09-1mg Mg: 1 mg $3,360.00 + -
100-09AF-5ug Animal-Free Mini: 5 ug $69.00 + -
100-09AF-20ug Animal-Free Std.: 20 ug $174.00 + -
100-09AF-100ug Animal-Free Value: 100 ug $436.00 + -
100-09AF-500ug Animal-Free Midgi: 500 ug $2,142.00 + -
100-09AF-1mg Animal-Free Mg: 1 mg $3,570.00 + -

Alternate Names:  B-cell stimulatory factor-1, BSF-1, BCDF, BCGF              

Accession Number: P05112
Description:  Interleukin 4 (IL-4) is an immunomodulatory cytokine that functions to induce naïve helper T cells to differentiate into type 2 T helper (Th2) cells. Th2 cells subsequently produce more IL-4 in a positive feedback loop. IL-4 also promotes immunoglobulin IgG to IgE isotype switching on B cells. IL-4 binds the IL-4Rα receptor to activate STAT6 signaling.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 15.1 kDa (130 aa)
AA Sequence:  MHKCDITLQE IIKTLNSLTE QKTLCTELTV TDIFAASKNT TEKETFCRAA TVLRQFYSHH EKDTRCLGAT AQQFHRHKQL IRFLKRLDRN LWGLAGLNSC PVKEANQSTL ENFLERLKTI MREKYSKCSS
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

TF-1 cell proliferation

≤250 pg/mL; ≥ 4.0 x 10^6 units/mg

 

**** International Units: The specific activity of Human IL-4 is approximately 1.4 x 10^4 IU/μg, which is calibrated against recombinant human IL-4 WHO International Standard (NIBSC code: 88/656). 

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

 

To receive more information or for a bulk quote, email quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 1011 Lot 0313
SDS Lot 0313  
PDS Animal-Free Lot 0313 Animal-Free  
SDS Animal-Free    
Papers that use this product:
 
 
 
 

  2018 Apr 13;13(4):e0195313. doi: 10.1371/journal.pone.0195313. eCollection 2018. PMID 29652910

 2017 Nov 1;77(21):5938-5951. doi: 10.1158/0008-5472.CAN-17-1007. Epub 2017 Sep 15. PMID 28916655

Tethering IL2 to Its Receptor IL2Rβ Enhances Antitumor Activity and Expansion of Natural Killer NK92 Cells.


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