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Human IL-1RA / IL-1F3

Animal-Free (AF) proteins are identical to regular proteins and include additional documentation showing that they were produced with no materials of animal or human origin using ANIMAL-FREE purification processes.  Learn more HERE.

SKU Select size Price Buy
100-195-20ug Mini: 20 ug $60.00  
100-195-100ug Std.: 100 ug $150.00  
100-195-250ug Value: 250 ug $375.00  
100-195-500ug Midig: 500 ug $650.00  
100-195-1mg Mg: 1 mg $1000.00  
100-195AF-20ug Animal-Free Mini: 20 ug $65.00  
100-195AF-100ug Animal-Free Std.: 100 ug $165.00  
100-195AF-250ug Animal-Free Value: 250 ug $410.00  
100-195AF-500ug Animal-Free Midig: 500 ug $715.00  
100-195AF-1mg Animal-Free Mg: 1 mg $1100.00  
View the available lots and COAs for this product HERE

Catalog:  100-195 and 100-195AF

Product Description
Alternate Names:  IL1RN, IL-1F3                    Accession Number: P18510    
Description:  Interleukin-1 receptor antagonist (IL-1RA) binds the interleukin 1 receptor (IL-1R) to antagonize interleukin 1 alpha and beta signaling.  IL-1RA is secreted by immune cells, epithelial cells, hepatocytes, and adipocytes to inhibit IL-1-induced inflammation.  IL-1RA therapy is used to treat auto-inflammatory diseases such as diabetes and rheumatoid arthritis. 
Source:  Genetically modified E.coli
Predicted MW:  17.3 kDa (with 153 amino acids)
AA Sequence:  MRPSGRKSSK MQAFRIWDVN QKTFYLRNNQ LVAGYLQGPN VNLEEKIDVV PIEPHALFLG IHGGKMCLSC VKSGDETRLQ LEAVNITDLS ENRKQDKRFA FIRSDSGPTT SFESAACPGW FLCTAMEADQ PVSLTNMPDE GVMVTKFYFQ EDE
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each production lot on its corresponding COA. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS-PAGE

≥ 95%

Endotoxin

Kinetic LAL (50% confidence)

≤ 1 EUs/µg

Biological Activity

Inhibition of human IL-1 alpha induced proliferation of D10.G4.1 cells

ED50 ≤ 50 ng/mL

(≥ 2.0 x 10^4 units/mg)


Product Preparation and Storage

Country of Origin:  USA         Shipping:  Room temperature               Storage Prior to Reconstitution:  -20°C

Product Reconstitution:  Sterile water at 0.1 mg/mL    

Instructions:  Centrifuge vial before opening.  Suspend the product by gently pipetting the above recommended solution down the sides of the vial.  DO NOT VORTEX.  Allow several minutes for complete reconstitution.  For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.   


Expiration Date:                      

12 months from date of receipt when stored at -20°C to-80°C as supplied. 

1 month when stored at 4°C after reconstituting as directed.

3 months when stored at -20°C to -80°C after reconstituting as directed.

                                                                                   

THIS PRODUCT IS FOR RESEARCH USE ONLY AND IS NOT FOR USE IN HUMANS!           

Data Sheet (PDF)

SDS Document (PDF)

Animal-Free Documents:

Data Sheet AF (PDF)

SDS Document AF (PDF)

To receive more information or a quote, complete our bulk/custom order form.

  • Select size
    Mini: 20 ug, Std.: 100 ug, Value: 250 ug, Midig: 500 ug, Mg: 1 mg, Animal-Free Mini: 20 ug, Animal-Free Std.: 100 ug, Animal-Free Value: 250 ug, Animal-Free Midig: 500 ug, Animal-Free Mg: 1 mg