Human IL-10

Interleukin 10 (IL-10) is an anti-inflammatory cytokine produced by macrophages and type 2 T helper (Th2) cells. IL-10 inhibits the production of pro-inflammatory cytokines such as interferon gamma (IFN-ɣ), tumor necrosis factor alpha (TNF-α), interleukin 2 (IL-2), interleukin 3 (IL-3), interleukin 4 (IL-4), and granulocyte-macrophage colony-stimulating factor (GM-CSF), made by macrophages and regulatory T cells. IL-10 also suppresses antigen presentation on antigen presenting cells, and enhances the survival, proliferation, and antibody production of B cells. Human IL-10 can act on murine cells, while murine IL-10 is not active on human cells.

SKU Select size Price Buy
100-83-2ug Mini: 2 ug $63.00 + -
100-83-10ug Std.: 10 ug $158.00 + -
100-83-100ug Value: 100 ug $609.00 + -
100-83-500ug Midgi: 500 ug $3,024.00 + -
100-83-1mg Mg: 1 mg $5,040.00 + -
100-83AF-2ug Animal-Free Mini: 2 ug $69.00 + -
100-83AF-10ug Animal-Free Std.: 10 ug $174.00 + -
100-83AF-100ug Animal-Free Value: 100 ug $667.00 + -
100-83AF-500ug Animal-Free Midgi: 500 ug $3,329.00 + -
100-83AF-1mg Animal-Free Mg: 1 mg $5,544.00 + -
Alternate Names:  B-TCGF, CSIF, TGIF               Accession Number: P22301
Description:  Interleukin 10 (IL-10) is an anti-inflammatory cytokine produced by macrophages and type 2 T helper (Th2) cells. IL-10 inhibits the production of pro-inflammatory cytokines such as interferon gamma (IFN-ɣ), tumor necrosis factor alpha (TNF-α), interleukin 2 (IL-2), interleukin 3 (IL-3), interleukin 4 (IL-4), and granulocyte-macrophage colony-stimulating factor (GM-CSF), made by macrophages and regulatory T cells. IL-10 also suppresses antigen presentation on antigen presenting cells, and enhances the survival, proliferation, and antibody production of B cells. Human IL-10 can act on murine cells, while murine IL-10 is not active on human cells.
Source:  Genetically modified E.coli.
Predicted MW: Noncovalent Homodimer, 18.8 kDa (161 aa)
AA Sequence:  MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

MC/9 cell Proliferation

≤5 ng/mL

 *** International Units: The specific activity of Human IL-10 is 5.4 x 10^3 IU/μg, which is calibrated against recombinant human IL-10 WHO International Standard (NIBSC code: 93/722).

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 1013 Lot 1013
SDS Lot 1013 Animal-Free  
PDS Animal-Free    
SDS Animal-Free    

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