Human IFN-gamma

Interferon gamma (IFN-γ) is a type II interferon that is critical during adaptive and innate immune responses to infection. IFN-γ is produced by T cells and natural killer cells following antigen-specific activation. IFN-γ binds IFN-γ receptors (IFN-γ R1 and IFN-γ R2), which are expressed on most immune cells, to activate the JAK-STAT pathway. IFN-γ-induced signaling increases the expression of class 1 major histocompatibility complex (MHC) molecules. Human IFN-γ is not cross-reactive with mouse IFN-γ.

SKU Select size Price Buy
100-77-20ug Mini: 20 ug $63.00 + -
100-77-100ug Std.: 100 ug $158.00 + -
100-77-250ug Value: 250 ug $363.00 + -
100-77-500ug Midgi: 500 ug $604.00 + -
100-77-1mg Mg: 1 mg $1,050.00 + -
100-77AF-20ug Animal-Free Mini: 20 ug $69.00 + -
100-77AF-100ug Animal-Free Std.: 100 ug $174.00 + -
100-77AF-250ug Animal-Free Value: 250 ug $399.00 + -
100-77AF-500ug Animal-Free Midgi: 500 ug $662.00 + -
100-77AF-1mg Animal-Free Mg: 1 mg $1,155.00 + -
Alternate Names:  Type II interferon, T cell interferon, Immune Interferon, MAF               Accession Number: P01579
Description:  Interferon gamma (IFN-γ) is a type II interferon that is critical during adaptive and innate immune responses to infection. IFN-γ is produced by T cells and natural killer cells following antigen-specific activation. IFN-γ binds IFN-γ receptors (IFN-γ R1 and IFN-γ R2), which are expressed on most immune cells, to activate the JAK-STAT pathway. IFN-γ-induced signaling increases the expression of class 1 major histocompatibility complex (MHC) molecules. Human IFN-γ is not cross-reactive with mouse IFN-γ.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 16.9 kDa (144 aa)
AA Sequence:  MQDPYVKEAE NLKKYFNAGH SDVADNGTLF LGILKNWKEE SDRKIMQSQI VSFYFKLFKN FKDDQSIQKS VETIKEDMNV KFFNSNKKKR DDFEKLTNYS VTDLNVQRKA IHELIQVMAE LSPAAKTGKR KRSQMLFQGR RASQ
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

Viral CPE assay using EMC virus on A549 cells

≥ 1.0 x 10^7 units/mg

 
Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date                      
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0108 Lot 0515
SDS Lot 0515 Lot 0519
PDS Animal-Free Lot 0515 Animal-Free   
SDS Animal-Free    

 

 

Papers that use this product:
 
 
 
Nature Cell 2020 May;22(5):591-602.  doi: 10.1038/s41556-020-0495-y. Epub 2020 Apr 13. PMID 32284542

Immunity 2019 Sep 17;51(3):451-464.e6.  doi: 10.1016/j.immuni.2019.07.007. Epub 2019 Aug 27. PMID 31471108

Differential Activation of the Transcription Factor IRF1 Underlies the Distinct Immune Responses Elicited by Type I and Type III Interferons

 2017 Apr;314:63-72. doi: 10.1016/j.cellimm.2017.02.005. Epub 2017 Feb 22. PMID 28242024

Enhancement of macrophage inflammatory responses by CCL2 is correlated with increased miR-9 expression and down regulation of the ERK1/2 phosphatase Dusp6.


 2017 Jun 21;2(3). pii: e00233-17. doi: 10.1128/mSphereDirect.00233-17. eCollection 2017 May-Jun. PMID 28656178

Gene Knockout Shows That PML (TRIM19) Does Not Restrict the Early Stages of HIV-1 Infection in Human Cell Lines.


Am J Physiol Endocrinol Metab 2015 Oct 15;309(8):E715-26.doi: 10.1152 / ajpendo.00153.2015.  Epub 2015 Aug 25. PMID 26306596

IL-1β Reciprocally Regulates Chemokine and Insulin Secretion in Pancreatic β-Cells via NF-κB

 2014 Jan 15;306(2):E131-49. doi: 10.1152/ajpendo.00347.2013. Epub 2013 Nov 26. PMID 24280128

NF-κB and STAT1 control CXCL1 and CXCL2 gene transcription.

 
 
 
 
 
 

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