Human GM-CSF

Granulocyte-macrophage colony-stimulating factor (GM-CSF) is hematopoietic growth factor produced by endothelial cells, monocytes, fibroblasts, and T cells. GM-CSF stimulates the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF promotes immune system development and regulates neutrophil function during infection. Human and mouse GM-CSF show no cross-reactivity.

SKU Select size Price Buy
100-08-5ug Mini: 5 ug $63.00 + -
100-08-20ug Std.: 20 ug $158.00 + -
100-08-100ug Value: 100 ug $394.00 + -
100-08-500ug Midgi: 500 ug $1,764.00 + -
100-08-1mg Mg: 1 mg $2,940.00 + -
100-08AF-5ug Animal-Free Mini: 5 ug $69.00 + -
100-08AF-20ug Animal-Free Std.: 20 ug $174.00 + -
100-08AF-100ug Animal-Free Value: 100 ug $436.00 + -
100-08AF-500ug Animal-Free Midgi: 500 ug $1,943.00 + -
100-08AF-1mg Animal-Free Mg: 1 mg $3,234.00 + -

Alternate Names:  CSF-2, Pluripoietin-α, MGI-1GM               Accession Number: P04141
Description:  Granulocyte-macrophage colony-stimulating factor (GM-CSF) is hematopoietic growth factor produced by endothelial cells, monocytes, fibroblasts, and T cells. GM-CSF stimulates the production of neutrophilic granulocytes, macrophages, and mixed granulocyte-macrophage colonies from bone marrow cells. GM-CSF promotes immune system development and regulates neutrophil function during infection. Human and mouse GM-CSF show no cross-reactivity.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 14.6 kDa (128 aa)
AA Sequence:  MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF DCWEPVQE
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

TF-1 cell proliferation

≤200 pg/mL; ≥ 5.0 x 10^6 units/mg

  *** International Units: The specific activity of Human GM-CSF is 1.6 x 10^4 IU/μg, which is calibrated against recombinant human GM-CSF WHO International Standard (NIBSC code: 88/646).

 Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

 

To receive more information or for a bulk quote, email quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0617 Lot 0617
SDS Lot 0608  
PDS Animal-Free Lot 0617 Animal-Free  
SDS Animal-Free    
     
Papers that use this product:
 
 
 
Acta Pharmacol Sin 2020 May 26.  doi: 10.1038/s41401-020-0412-8. Online ahead of print. PMID 32457418
Sci Rep2020 Apr 14;10(1):6335.  doi: 10.1038/s41598-020-63346-4. PMID 32286456
 2019 May 23. pii: haematol.2018.212886. doi: 10.3324/haematol.2018.212886. [Epub ahead of print] PMID 31123028

The novel Isatin analog KS99 targets stemness markers in acute myeloid leukemia.


 

 2018 Apr 13;13(4):e0195313. doi: 10.1371/journal.pone.0195313. eCollection 2018. PMID 29652910

Elderly dendritic cells respond to LPS/IFN-γ and CD40L stimulation despite incomplete maturation.


 2017 Jun 1;127(6):2339-2352. doi: 10.1172/JCI92217. Epub 2017 May 2. PMID 28463230

CD1b-autoreactive T cells contribute to hyperlipidemia-induced skin inflammation in mice.


 2017 Jun 22;129(25):3344-3351. doi: 10.1182/blood-2016-11-750729. Epub 2017 Apr 13. PMID 28408459

EPCR expression marks UM171-expanded CD34+ cord blood stem cells.


 2016 May;97(5):1077-1086. doi: 10.1099/jgv.0.000441. Epub 2016 Mar 1. PMID 26932515

Characterization of Nipah virus infection in a model of human airway epithelial cells cultured at an air-liquid interface.


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