Human FLT-3 Ligand Gel
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Human FLT-3 Ligand

Animal-Free (AF) proteins are identical to regular proteins and include additional documentation showing that they were produced with no materials of animal or human origin using ANIMAL-FREE purification processes.  Learn more HERE.

SKU Select size Price Buy
100-21-2ug Mini: 2 ug $63.00 + -
100-21-10ug Std.: 10 ug $158.00 + -
100-21-100ug Value: 100 ug $457.00 + -
100-21-500ug Midgi: 500 ug $2268.00 + -
100-21-1mg Mg: 1 mg $3780.00 + -
100-21AF-2ug Animal-Free Mini: 2 ug $69.00 + -
100-21AF-10ug Animal-Free Std.: 10 ug $174.00 + -
100-21AF-100ug Animal-Free Value: 100 ug $499.00 + -
100-21AF-500ug Animal-Free Midgi: 500 ug $2494.00 + -
100-21AF-1mg Animal-Free Mg: 1 mg $4158.00 + -
  • Weight
    0.0100 lbs
  • SKU
    100-21-2ug
Alternate Names:  Flt3L, Fms-related tyrosine kinase 3 ligand
Accession Number: P49771
Description:  Fms-related tyrosine kinase 3 ligand (FLT-3 ligand) is a growth factor that regulates hematopoietic cell proliferation. FLT-3 ligand signalling is transmitted through the fms-related tyrosine kinase 3 (FLT-3) receptor. FLT-3 ligand promotes the long-term expansion and differentiation of pro-B cells in the presence of interleukin 7 (IL-7) or in combination of IL-7 and interleukin 3 (IL-3). Human FLT-3 ligand is active on mouse cells.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 17.7 kDa (155 aa)
AA Sequence:  MTQDCSFQHS PISSDFAVKI RELSDYLLQD YPVTVASNLQ DEELCGGLWR LVLAQRWMER LKTVAGSKMQ GLLERVNTEI HFVTKCAFQP PPSCLRFVQT NISRLLQETS EQLVALKPWI TRQNFSRCLE LQCQPDSSTL PPPWSPRPLE ATAPT
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM NaCl, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

OCI-AML5 cell proliferation

≤10 ng/mL; ≥ 1.0 x 10^5 units/mg

(typical ED50 is 1-3 ng/mL)

In addition to the acceptance criteria, this product has been tested with the following assay:
(1)  Production of IL-6 by human PBMCs (typical ED50 is 50-150 ng/mL)

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or a quote, complete our bulk/custom order form.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0817 Lot 0317
SDS Lot 0717 Lot 0519
PDS Animal-Free Lot 0317 Lot 0817
SDS Animal-Free Lot 0817 Animal-Free  
  Lot 0717 Animal-Free  
  Lot 0317 Animal-Free  
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