Human FGF-basic 154 aa (FGF2) Gel
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Human FGF-basic 154 aa (FGF2)

Human recombinant FGF-basic 154 aa or FGF-2 (FGF2) protein is a recombinant protein widely used as a growth factor in many cell culture applications.  Recombinant FGF-2 is a critical growth factor needed for expansion and long term maintenance of pluripotent stem cell culture.

Tested for biological activity in a 3T3 cell proliferation assay. Lyophilized protein, high purity, low endotoxin, high quality FGF-2 is manufactured in the USA by protein manufacturer Shenandoah and available for sale in bulk amounts.  Lot-specific results are reported for each production lot.  

SKU Select size Price Buy
100-146-10ug Mini: 10 ug $63.00 + -
100-146-50ug Std.: 50 ug $158.00 + -
100-146-100ug Value: 100 ug $226.00 + -
100-146-500ug Midgi: 500 ug $483.00 + -
100-146-1mg Mg: 1 mg $840.00 + -
100-146AF-10ug Animal-Free Mini: 10 ug $69.00 + -
100-146AF-50ug Animal-Free Std.: 50 ug $174.00 + -
100-146AF-100ug Animal-Free Value: 100 ug $247.00 + -
100-146AF-500ug Animal-Free Midgi: 500 ug $531.00 + -
100-146AF-1mg Animal-Free Mg: 1 mg $893.00 + -
  • Weight
    0.0100 lbs
  • SKU
    100-146-10ug

Alternate Names:  FGF2, HBGF-2, Prostatropin              

Accession Number: P09038

Description:  Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis.  FGF-basic also has cardioprotective functions during heart injury.  FGF-basic is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state.  Recombinant FGF-basic 154 is the full length FGF-basic protein encoded by the human FGF-2 gene.  FGF-basic 154 is the most popular tissue culture product at Shenandoah Biotechnology, Inc.  There are no detectable differences in biological activity between FGF-basic 154 and the truncated FGF-basic 147 proteins.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 17.3 kDa (155 aa)
AA Sequence:  MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 75 mM sodium chloride, pH 8.0

FGF-2 Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

3T3 Proliferation

≤5 ng/mL; ≥ 2.0 x 10^5 units/mg

(typical ED50 is < 1 ng/mL)

Preparation and Storage

Country of Origin:  USA       

Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C

Product Reconstitution:  Sterile water at 0.1 mg/mL

Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

 

Expiration Date:                      

12 months from date of receipt when stored at -20°C to -80°C as supplied.

1 month when stored at 4°C after reconstituting as directed.

3 months when stored at -20°C to -80°C after reconstituting as directed.

 

To receive more information or a quote, complete our bulk/custom order form.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0213 Lot 0918
SDS   Lot 1018
PDS Animal-Free   Lot 09191
SDS Animal-Free    
Papers that use this product:
 
 
Nat Med2020 Apr 27. doi: 10.1038/s41591-020-0821-8. Online ahead of print.

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Targeting Hyaluronan Interactions for Glioblastoma Stem Cell Therapy.


 2019 Jan 2;5(1):eaat0456. doi: 10.1126/sciadv.aat0456. eCollection 2019 Jan.

IDH3α regulates one-carbon metabolism in glioblastoma.


 2019 Jan 10;4(1). pii: 122933. doi: 10.1172/jci.insight.122933. [Epub ahead of print]

Combined functional genomic and chemical screens identify SETD8 as a therapeutic target in MYC-driven medulloblastoma.

Veo B1Danis E1Pierce A1,2Sola I1Wang D1Foreman NK1,2,3Jin J4Ma A4Serkova N5Venkataraman S1,2Vibhakar R1,2,3,6.

 2011 Mar 15;108(11):4453-8. doi: 10.1073/pnas.1101657108. Epub 2011 Mar 1.

Hedgehog-responsive candidate cell of origin for diffuse intrinsic pontine glioma.


 2012 Jul;108(3):395-402. doi: 10.1007/s11060-012-0842-3. Epub 2012 Mar 2.

Expression of epidermal growth factor variant III (EGFRvIII) in pediatric diffuse intrinsic pontine gliomas.


 2015 Nov;77(5):794-802; discussion 802. doi: 10.1227/NEU.0000000000000918.

Neural Placode Tissue Derived From Myelomeningocele Repair Serves as a Viable Source of Oligodendrocyte Progenitor Cells.


 2018 May;20(5):432-442. doi: 10.1016/j.neo.2018.02.004. Epub 2018 Mar 22.

A Pre-Clinical Assessment of the Pan-ERBB Inhibitor Dacomitinib in Pediatric and Adult Brain Tumors.


 2017 Sep 28;549(7673):533-537. doi: 10.1038/nature24014. Epub 2017 Sep 20.

Targeting neuronal activity-regulated neuroligin-3 dependency in high-grade glioma.


 2017 May 8;31(5):635-652.e6. doi: 10.1016/j.ccell.2017.03.011. Epub 2017 Apr 20.

Transcriptional Dependencies in Diffuse Intrinsic Pontine Glioma.


 2015 Jul 13;10(7):e0132823. doi: 10.1371/journal.pone.0132823. eCollection 2015.

ROCK Inhibition Facilitates In Vitro Expansion of Glioblastoma Stem-Like Cells.


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