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Canine IL-4

Animal-Free (AF) proteins are identical to regular proteins and include additional documentation that they were produced using ANIMAL-FREE PROCESSES and contain only ANIMAL-FREE RAW MATERIALS.  Learn more HERE.

SKU Select size Price Buy
400-09-5ug Mini: 5 ug $60.00  
400-09-25ug Std.: 25 ug $150.00  
400-09-100ug Value: 100 ug $550.00  
400-09-500ug Midgi: 500 ug $1650.00  
400-09-1mg Mg: 1 mg $3000.00  
400-09AF-5ug Animal-Free Mini: 5 ug $65.00  
400-09AF-25ug Animal-Free Std.: 25 ug $165.00  
400-09AF-100ug Animal-Free Value: 100 ug $605.00  
400-09AF-500ug Animal-Free Midgi: 500 ug $1815.00  
400-09AF-1mg Animal-Free Mg: 1 mg $3300.00  
View the available lots and COAs for this product HERE

Catalog Number: 400-09 and 400-09AF

Product Description
Alternate Names:  B-cell stimulatory factor-1, BSF-1, BCDF, BCGF                 Accession Number: O77762   
Description:  Interleukin 4 (IL-4) is an immunomodulatory cytokine that functions to induce naïve helper T cells to differentiate into type 2 T helper (Th2) cells.  Th2 cells subsequently produce more IL-4 in a positive feedback loop.  IL-4 also promotes immunoglobulin IgG to IgE isotype switching on B cells.  IL-4 binds the IL-4Rα receptor to activate STAT6 signaling. 
Source:  Genetically modified E.coli
Predicted MW:  Monomer, 12.9 kDa (with 109 amino acids)
AA Sequence:  MHNFNITIKE IIKMLNILTA RNDSCMELTV KDVFTAPKNT SDKEIFCRAA TVLRQIYTHN CSNRYLRGLY RNLSSMANKT CSMNEIKKST LKDFLERLKV IMQKKYYRH
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)

Product Specifications*
*Lot-specific values for the following specifications are supplied with each production lot on its corresponding COA. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS-PAGE

≥95%

Endotoxin

Kinetic LAL (50% confidence)

≤ 1 EUs/µg

Biological Activity

TF-1 cell proliferation

ED50 ≤ 25 ng/mL (≥ 4.0 x 10^4 units/mg)


Product Preparation and Storage
Country of Origin:  USA         Shipping:  Room temperature              
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL    
Instructions:  Centrifuge vial before opening.  Suspend the product by gently pipetting the above recommended solution down the sides of the vial.  DO NOT VORTEX.  Allow several minutes for complete reconstitution.  For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.   


Expiration Date:                      

12 months from date of receipt when stored at -20°C to -80°C as supplied. 

1 month when stored at 4°C after reconstituting as directed.

3 months when stored at -20°C to -80°C after reconstituting as directed.

                                                                                   

 

Data Sheet (PDF)

SDS Document (PDF)

Animal-Free Documents:

Data Sheet AF (PDF)

SDS Document AF (PDF)

To receive more information or a quote, complete our bulk/custom order form.

  • Select size
    Mini: 5 ug, Std.: 25 ug, Value: 100 ug, Midgi: 500 ug, Mg: 1 mg, Animal-Free Mini: 5 ug, Animal-Free Std.: 25 ug, Animal-Free Value: 100 ug, Animal-Free Midgi: 500 ug, Animal-Free Mg: 1 mg