Basic E.coli System Expression Package

Included in the package:

Steps in the process: Deliverables:
1. Cloning gene of interest with 1 tag of choice (GST or 6XHis) ✓ 2 ug DNA
✓ Sequence chromatogram
2. Sub-cloning gene into expression vector  
3. Expression and Purification (>90%) ✓ 1 mg of protein
✓ SDS-PAGE image
Additional options (additional charges via quotation):
Protein sequence validation with mass spec ✓ Mass spec trace report
Endotoxin analysis ✓ LAL endotoxin report (Units/ug protein)
Additional purified material ✓ Available in increments of 1 mg or more
Product manipulations (tag cleavage, desalting, aliquoting, lyophilization, endotoxin removal)  
Time frame is 4-6 weeks for the package without additional options.

 

Expression Capabilities: Fermentation volumes that scale to 5L, 20L, and 80L

Purification Capabilities:

  • Ion Exchange (S-Sepharose, CM-Sepharose, Q-Sepharose, DEAE Sepharose)
  • Hydrophobic Interaction Chromatography (Butyl Sepharose, Phenyl Sepharose)
  • Hydroxyapatite Chromatography
  • Heparin Purification
  • Affinity Purification