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Mouse IL-3

 Interleukin 3 (IL-3) is a cytokine that is produced by activated T cells and mast cells. IL-3 induces the differentiation of hematopoietic stem cells into myeloid precursor cells, such as erythrocyte, megakaryocyte, granulocyte, monocyte, and dendritic cells. IL-3 also functions in the nervous system and is important during the B-1 cell regulation of chronic inflammatory diseases.

SKU Select size Price Buy
200-01-2ug Mini: 2 ug $63.00 + -
200-01-10ug Std.: 10 ug $158.00 + -
200-01-100ug Value: 100 ug $510.00 + -
200-01-500ug Midgi: 500 ug $1,701.00 + -
200-01-1mg Mg: 1 mg $2,835.00 + -
200-01AF-2ug Animal-Free Mini: 2 ug $69.00 + -
200-01AF-10ug Animal-Free Std.: 10 ug $174.00 + -
200-01AF-100ug Animal-Free Value: 100 ug $562.00 + -
200-01AF-500ug Animal-Free Midgi: 500 ug $1,869.00 + -
200-01AF-1mg Animal-Free Mg: 1 mg $3,119.00 + -
Alternate Names:  MCGF, Multi-CSF, HCGF, P-cell stimulating factor                
Accession Number: P01586
Description:  Interleukin 3 (IL-3) is a cytokine that is produced by activated T cells and mast cells. IL-3 induces the differentiation of hematopoietic stem cells into myeloid precursor cells, such as erythrocyte, megakaryocyte, granulocyte, monocyte, and dendritic cells. IL-3 also functions in the nervous system and is important during the B-1 cell regulation of chronic inflammatory diseases.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 15.2 kDa (135 aa)
AA Sequence:  MDTHRLTRTL NCSSIVKEII GKLPEPELKT DDEGPSLRNK SFRRVNLSKF VESQGEVDPE DRYVIKSNLQ KLNCCLPTSA NDSALPGVFI RDLDDFRKKL RFYMVHLNDL ETVLTSRPPQ PASGSVSPNR GTVEC
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA)

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

M-NFS-60 cell proliferation

≤250 pg/mL

 
Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0613 Lot 1008
SDS Lot 1008 Lot 0613
PDS Animal-Free Lot 0613 Animal-Free  
SDS Animal-Free    

 

 

 

Papers that cite use of this product:
 
 
 
 2018 Mar;48(3):288-296. doi: 10.1111/cea.13075. Epub 2018 Jan 22. PMID 29247574

IgE promotes type 2 innate lymphoid cells in murine food allergy.


 2016 Oct 27;7:13198. doi: 10.1038/ncomms13198.  PMID 27786273

The Nedd4-2/Ndfip1 axis is a negative regulator of IgE-mediated mast cell activation.


 2015 Aug 15;195(4):1377-87. doi: 10.4049/jimmunol.1302874. Epub 2015 Jul 1. PMID 26136426

Tetraspanin CD151 Is a Negative Regulator of FcεRI-Mediated Mast Cell Activation.

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