Human TGF beta 1
Transforming growth factor-beta 1 (TGF-b1) is a member of the TGF-beta superfamily of cytokines. Members of this family exhibit regulatory activity in immunity, proliferation, adhesion, migration, and growth inhibition pathways.
Alternate Names: Differentiation inhibiting factor, cartilage-inducing factor Accession Number: P01137
Description: Transforming growth factor-beta 1 (TGF-b1) is a member of the TGF-beta superfamily of cytokines. Members of this family exhibit regulatory activity in immunity, proliferation, adhesion, migration, and growth inhibition pathways. TGF-b1 signals through SMAD proteins via the TGF-bRI and TGF-bRII receptors. TGF-b1 is often used in addition to recombinant IL-2 for production of Treg cells in cell culture.
Source: Genetically modified CHO cells.
Predicted MW: Dimer, 12.8/25.6 kDa (112/224 aa)
AA Sequence: ALDTNYCFSS TEKNCCVRQL YIDFRKDLGW KWIHEPKGYH ANFCLGPCPY IWSLDTQYSK VLALYNQHNP GASAAPCCVP QALEPLPIVY YVGRKPKVEQ LSNMIVRSCK CS
Formulation: Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1 % trifluoroacetic acid (TFA) and Trehalose in a 20:1 Trehalose to protein ratio
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA. The values provided here are minimum expected values to pass internal requirements.
Method of Determination
Reducing and Non-Reducing SDS PAGE
Inhibition of IL-4-induced HT-2 cell proliferation
**** Reference Units: The activity of Human TFG-beta is 2.2 x 10^4 RU/μg, which was calibrated against recombinant human TGF-beta 1 NIBSC code: 89/514.
Preparation and Storage
Country of Origin: USA
Shipping: Room temperature
Storage Prior to Reconstitution: -20°C
Product Reconstitution: Sterile 10 mM HCl at 0.1 mg/mL
Instructions: Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.
- 12 months from date of receipt when stored at -20°C to -80°C as supplied.
- 1 month when stored at 4°C after reconstituting as directed.
- 3 months when stored at -20°C to -80°C after reconstituting as directed.
|Product Datasheets||Certificates of Analysis||Certificate of Origin|
|PDS||Lot 1118||Lot 0118|
|SDS||Lot 0418||Lot 1016|
|Lot 0118||Lot 0816|
|Lot 0316 without BSA||Lot 0710|
|Lot 0316 with BSA||Lot 0615|
|Lot 0615||Lot 0614|
|Lot 0710||Lot 0517|
|Lot 0614||Lot 0418|
|Lot 1016||Lot 0318|
|Lot 0816||Lot 0316|
|Lot 0517||Lot 0218|
|Lot 0318||Lot 0118|
|Lot 0218||Lot 0519|
Potent inhibition of tumour cell proliferation and immunoregulatory function by mitochondria-targeted atovaquone
Hectd3 promotes pathogenic Th17 lineage through Stat3 activation and Malt1 signaling in neuroinflammation.
Tissue microenvironment initiates an immune response to structural components of Staphylococcus aureus.
TGF-β Requires the Activation of Canonical and Non-Canonical Signalling Pathways to Induce Skeletal Muscle Atrophy
Cancer Immunol Immunother. 2017 Jun;66(6):737-751. doi: 10.1007/s00262-017-1965-3. Epub 2017 Mar 9. PMID 28280853