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Human G-CSF

Granulocyte-colony stimulating factor (G-CSF) is a cytokine that functions as a potent inducer of neutrophilic granulocyte proliferation, terminal differentiation, and activation.  G-CSF synthesis occurs in monocyte, macrophage, epithelial, endothelial, and fibroblast cells after activation by bacterial endotoxins, tumor necrosis factor alpha (TNF-α), interleukin 1 (IL-1), or interleukin 17 (IL-17).  The functional activity of G-CSF is mediated through the granulocyte colony-stimulating factor receptor (G-CSF-R) to activate JAK/STAT and MAPK signal transduction pathways.  G-CSF also promotes neurogenesis and inhibits neuronal apoptosis.  Human and mouse G-CSF proteins are cross-reactive. 

SKU Select size Price Buy
100-72-2ug Mini: 2 ug $63.00 + -
100-72-10ug Std.: 10 ug $158.00 + -
100-72-100ug Value: 100 ug $510.00 + -
100-72-500ug Midgi: 500 ug $2,205.00 + -
100-72-1mg Mg: 1 mg $3,675.00 + -
100-72AF-2ug Animal-Free Mini: 2 ug $69.00 + -
100-72AF-10ug Animal-Free Std.: 10 ug $174.00 + -
100-72AF-100ug Animal-Free Value: 100 ug $562.00 + -
100-72AF-500ug Animal-Free Midgi: 500 ug $2,426.00 + -
100-72AF-1mg Animal-Free Mg: 1 mg $4,043.00 + -
Alternate Names:  CSF-3, MGI-1G, GM-CSFβ, pluripoietin              
Accession Number: P09919
Description:  Granulocyte-colony stimulating factor (G-CSF) is a cytokine that functions as a potent inducer of neutrophilic granulocyte proliferation, terminal differentiation, and activation.  G-CSF synthesis occurs in monocyte, macrophage, epithelial, endothelial, and fibroblast cells after activation by bacterial endotoxins, tumor necrosis factor alpha (TNF-α), interleukin 1 (IL-1), or interleukin 17 (IL-17).  The functional activity of G-CSF is mediated through the granulocyte colony-stimulating factor receptor (G-CSF-R) to activate JAK/STAT and MAPK signal transduction pathways.  G-CSF also promotes neurogenesis and inhibits neuronal apoptosis.  Human and mouse G-CSF proteins are cross-reactive. 
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 18.8 kDa (175 aa)
AA Sequence:  MTPLGPASSL PQSFLLKCLE QVRKIQGDGA ALQEKLCATY KLCHPEELVL LGHSLGIPWA PLSSCPSQAL QLAGCLSQLH SGLFLYQGLL QALEGISPEL GPTLDTLQLD VADFATTIWQ QMEELGMAPA LQPTQGAMPA FASAFQRRAG GVLVASHLQS FLEVSYRVLR HLAQP
Formulation:  -  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 20 mM acetic acid, 50 mM sodium chloride.

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

NFS-60 cell proliferation

≤60 pg/mL

 **** International Units: The specific activity of Human G-CSF is approximately 5.4 x 10^4 IU/μg, which is calibrated against recombinant human G-CSF WHO International Standard (NIBSC code: 09/136). 

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date                      
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0618 Lot 0515
SDS Lot 0618 Animal-Free Lot 0618
PDS Animal-Free    
SDS Animal-Free    

 

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