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Human FGF-basic 154 aa (FGF2)

Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis.  FGF-basic also has cardioprotective functions during heart injury.  FGF-basic is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state.  Recombinant FGF-basic 154 is the full length FGF-basic protein encoded by the human FGF-2 gene.  FGF-basic 154 is the most popular tissue culture product at Shenandoah Biotechnology, Inc.  There are no detectable differences in biological activity between FGF-basic 154 and the truncated FGF-basic 147 proteins.

SKU Select size Price Buy
100-146-10ug Mini: 10 ug $63.00 + -
100-146-50ug Std.: 50 ug $158.00 + -
100-146-100ug Value: 100 ug $226.00 + -
100-146-500ug Midgi: 500 ug $483.00 + -
100-146-1mg Mg: 1 mg $840.00 + -
100-146AF-10ug Animal-Free Mini: 10 ug $69.00 + -
100-146AF-50ug Animal-Free Std.: 50 ug $174.00 + -
100-146AF-100ug Animal-Free Value: 100 ug $247.00 + -
100-146AF-500ug Animal-Free Midgi: 500 ug $531.00 + -
100-146AF-1mg Animal-Free Mg: 1 mg $893.00 + -

Alternate Names:  FGF2, HBGF-2, Prostatropin              

Accession Number: P09038

Description:  Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis.  FGF-basic also has cardioprotective functions during heart injury.  FGF-basic is a critical component for embryonic stem cell culture systems and is necessary for maintaining cells in an undifferentiated state.  Recombinant FGF-basic 154 is the full length FGF-basic protein encoded by the human FGF-2 gene.  FGF-basic 154 is the most popular tissue culture product at Shenandoah Biotechnology, Inc.  There are no detectable differences in biological activity between FGF-basic 154 and the truncated FGF-basic 147 proteins.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 17.3 kDa (155 aa)
AA Sequence:  MAAGSITTLP ALPEDGGSGA FPPGHFKDPK RLYCKNGGFF LRIHPDGRVD GVREKSDPHI KLQLQAEERG VVSIKGVCAN RYLAMKEDGR LLASKCVTDE CFFFERLESN NYNTYRSRKY TSWYVALKRT GQYKLGSKTG PGQKAILFLP MSAKS
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 75 mM sodium chloride, pH 8.0

FGF-2 Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

3T3 Proliferation

≤5 ng/mL; ≥ 2.0 x 10^5 units/mg

Preparation and Storage

Country of Origin:  USA       

Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C

Product Reconstitution:  Sterile water at 0.1 mg/mL

Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

 

Expiration Date:                      

12 months from date of receipt when stored at -20°C to -80°C as supplied.

1 month when stored at 4°C after reconstituting as directed.

3 months when stored at -20°C to -80°C after reconstituting as directed.

 

To receive more information or a bulk quote, email us at quotes@shenandoah-bt.com.

 

Papers that use this product:
 
 
July 2020 UC Santa Cruz. ProQuest ID: Sanders_ucsc_0036E_12024. Merritt ID: ark:/13030/m5102czj. Retrieved from https://escholarship.org/uc/item/8452n2gj
 
 
Sanders, L. M. (2020)
 

Nat Med2020 Apr 27. doi: 10.1038/s41591-020-0821-8. Online ahead of print. PMID 32341579

Locoregionally Administered B7-H3-targeted CAR T Cells for Treatment of Atypical Teratoid/Rhabdoid Tumors

 2019 Apr;12(1):47-56. doi: 10.1007/s12307-019-00224-2. Epub 2019 May 11. PMID 31079324

Targeting Hyaluronan Interactions for Glioblastoma Stem Cell Therapy.


 2019 Mar 4;10(1):1023. doi: 10.1038/s41467-019-08823-9. PMID 30833574

ACVR1 R206H cooperates with H3.1K27M in promoting diffuse intrinsic pontine glioma pathogenesis.


 2019 Jan 2;5(1):eaat0456. doi: 10.1126/sciadv.aat0456. eCollection 2019 Jan. PMID 30613765

IDH3α regulates one-carbon metabolism in glioblastoma.


 2019 Jan 10;4(1). pii: 122933. doi: 10.1172/jci.insight.122933. [Epub ahead of print] PMID 30626740

Combined functional genomic and chemical screens identify SETD8 as a therapeutic target in MYC-driven medulloblastoma.

Veo B1Danis E1Pierce A1,2Sola I1Wang D1Foreman NK1,2,3Jin J4Ma A4Serkova N5Venkataraman S1,2Vibhakar R1,2,3,6.


 2018 May;20(5):432-442. doi: 10.1016/j.neo.2018.02.004. Epub 2018 Mar 22. PMID 29574250

A Pre-Clinical Assessment of the Pan-ERBB Inhibitor Dacomitinib in Pediatric and Adult Brain Tumors.


 2017 Sep 28;549(7673):533-537. doi: 10.1038/nature24014. Epub 2017 Sep 20. PMID 28959975

Targeting neuronal activity-regulated neuroligin-3 dependency in high-grade glioma.


 2017 May 8;31(5):635-652.e6. doi: 10.1016/j.ccell.2017.03.011. Epub 2017 Apr 20. PMID 28434841

Transcriptional Dependencies in Diffuse Intrinsic Pontine Glioma.


 2015 Nov;77(5):794-802; discussion 802. doi: 10.1227/NEU.0000000000000918. PMID 26225855

Neural Placode Tissue Derived From Myelomeningocele Repair Serves as a Viable Source of Oligodendrocyte Progenitor Cells.


 2015 May;17(5):697-707. doi: 10.1093/neuonc/nou303. Epub 2014 Nov 2. PMID 25366336

Preclinical antitumor efficacy of selective exportin 1 inhibitors in glioblastoma.


 2015 Jan;23(1):99-107. doi: 10.1038/mt.2014.177. Epub 2014 Sep 9. PMID 25200130

Use of miRNA response sequences to block off-target replication and increase the safety of an unattenuated, glioblastoma-targeted oncolytic HSV.


 2015 Jul 13;10(7):e0132823. doi: 10.1371/journal.pone.0132823. eCollection 2015. PMID 26167936

ROCK Inhibition Facilitates In Vitro Expansion of Glioblastoma Stem-Like Cells.


 2013 Mar;21(3):561-9. doi: 10.1038/mt.2012.211. Epub 2012 Oct 16. PMID 23070115

Effective treatment of an orthotopic xenograft model of human glioblastoma using an EGFR-retargeted oncolytic herpes simplex virus.

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