Human R-Spondin 1

R-Spondin 1 (RSPO-1)is a secreted growth factor that stabilizes β-catenin and enhances WNT signaling.  RSPO-1 is expressed in many tissues and regulates female sex determination, XX gonad development, oocyte differentiation, and hematopoietic stem cell specification.

SKU Select size Price Buy
100-208-5ug Mini: 5 ug $63.00 + -
100-208-25ug Std.: 25 ug $158.00 + -
100-208-100ug Value: 100 ug $504.00 + -
100-208-500ug Midgi: 500 ug $1,575.00 + -
100-208-1mg Mg: 1 mg $3,150.00 + -
             
Alternate Names:  hRspo1, Roof plate-specific spondin-1                      Accession Number: Q2MKA7

Description:  R-Spondin 1 (RSPO-1)is a secreted growth factor that stabilizes β-catenin and enhances WNT signaling.  RSPO-1 is expressed in many tissues and regulates female sex determination, XX gonad development, oocyte differentiation, and hematopoietic stem cell specification.  The RSPO-1 stabilization of β-catenin promotes intestinal crypt cell proliferation.  This suggests that RSPO-1 functions as a stem cell growth factor and may have therapeutic potential in the treatment of gastrointestinal diseases and chemotherapy-induced intestinal mucositis.  Additionally, recombinant RSPO-1 is a critical protein used to establish 3D intestinal organoid cultures (stomach, small intestine, colon, pancreas, and liver sources).  Shenandoah is a manufacturer that offers an affordable option of recombinant RSPO-1 protein.
Source:  Genetically modified CHO cells. 
Predicted MW:  Monomer, 25.6 kDa (with 233 amino acids)
AA Sequence:  RISAEGSQAC AKGCELCSEV NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE HCEACFSHNF CTKCKEGLYL HKGRCYPACP EGSSAANGTM ECSSPAQCEM SEWSPWGPCS KKQQLCGFRR GSEERTRRVL HAPVGDHAAC SDTKETRRCT VRRVPCPEGQ KRRKGGQGRR ENANRNLARK ESKEAGAGSR RRKGQQQQQQ QGTVGPLTSA GPA
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing phosphate buffered saline (PBS).

RSPO-1 Product Specifications*
*Lot-specific values for the following specifications are supplied with each production lot on its corresponding COA. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS-PAGE

≥ 95%

Endotoxin

Kinetic LAL (50% confidence)

≤ 1 EUs/µg

Biological Activity

Luciferase induction in HEK-293 STF cells in the presence of Murine Wnt-3a

ED50 ≤ 50 ng/mL 

Preparation and Storage

Country of Origin:  USA

Shipping:  Room temperature

Storage Prior to Reconstitution:  -20°C

Product Reconstitution:  Sterile water at 0.1 mg/mL

Instructions:  Centrifuge vial before opening.  Suspend the product by gently pipetting the above recommended solution down the sides of the vial.  DO NOT VORTEX.  Allow several minutes for complete reconstitution.  For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C, and avoid repeat freeze thaws.

Expiration Date                   

12 months from date of receipt when stored at -20°C to -80°C as supplied.

1 month when stored at 4°C after reconstituting as directed.

3 months when stored at -20°C to -80°C after reconstituting as directed.

                                                                                   

THIS PRODUCT IS FOR RESEARCH USE ONLY AND IS NOT FOR USE IN HUMANS!

To receive more information or a bulk quote, email us at quotes@shenandoah-bt.com.

  

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 1018 Lot 1117
SDS Lot 1117  

 

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