Shenandoah Chemokines Will Make Your Cells,
"MOVE IT, MOVE IT!"
Chemotaxis of mammalian cells is important for a variety of functions related to:
- development (ex. fertilization of the egg)
- immunology (ex. lymphocyte recruitment)
- disease (ex. cancer metastasis)
The process of chemotaxis is initiated by a ligand/receptor interaction in which the signaling pathway activated by the receptor polarizes the cell to sense and move in the direction of increased ligand concentrations. Ligands that share a conserved three dimensional shape, characterized by disulfide bonds between cysteines, are called chemokines. The chemokine family activates signaling (thus, chemotaxis) through a group of receptors known as G-protein coupled receptors (GPCRs).
Shenandoah chemokines are rigorously tested for activity in migration assays using Boyden chambers and primary blood cells (PBMCs, purified T cells, etc) or cell lines (THP-1, Jurkat, etc).