Rat IFN-gamma

Interferon gamma (IFN-γ) is a type II interferon that is critical during adaptive and innate immune responses to infection.  IFN-γ is produced by T cells and natural killer cells following antigen-specific activation.

SKU Select size Price Buy
300-04-20ug Mini: 20 ug $63.00 + -
300-04-100ug Std.: 100 ug $158.00 + -
300-04-250ug Value: 250 ug $363.00 + -
300-04-500ug Midgi: 500 ug $604.00 + -
300-04-1mg Mg: 1 mg $1,050.00 + -

Alternate Names:  Type II interferon, T cell interferon, Immune Interferon, MAF               Accession Number: P01581
Description:  Interferon gamma (IFN-γ) is a type II interferon that is critical during adaptive and innate immune responses to infection.  IFN-γ is produced by T cells and natural killer cells following antigen-specific activation.  IFN-γ binds IFN-γ receptors (IFN-γ R1 and IFN-γ R2), which are expressed on most immune cells, to activate the JAK-STAT pathway.  IFN-γ-induced signaling increases the expression of class 1 major histocompatibility complex (MHC) molecules.  
Source:  Genetically modified E.coli.
Predicted MW: Noncovalently-linked homodimer, 15.6 / 31.2 kDa (135 / 270 aa) 
AA Sequence:  MQGTLIESLE SLKNYFNSSS MDAMEGKSLL LDIWRNWQKD GNTKILESQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT NFFSNSKAKK DAFMSIAKFE VNNPQIQHKA VNELIRVIHQ LSPESSLRKR KRSRC
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

No biological activity data is available at this time

No biological activity data is available at this time 

 
Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.  

Expiration Date                      
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or for a bulk quote, email us at quotes@shenandoah-bt.com.

 

 

Product Datasheets Certificates of Analysis
PDS Lot 1107
SDS  

 

Papers that use this product:
 
 
 
Am J Physiol Endocrinol Metab 2014 Jan 15;306(2):E131-49.  doi: 10.1152 / ajpendo .00347 .2013. Epub 2013 Nov 26. PMID 24280128

NF-κB and STAT1 Control CXCL1 and CXCL2 Gene Transcription

Cell Immunol 2011;271(2):379-84.  doi: 10.1016/j.cellimm.2011.08.004. Epub 2011 Aug 12. PMID 21885043

The Gene Encoding cyclooxygenase-2 Is Regulated by IL-1β and Prostaglandins in 832/13 Rat Insulinoma Cells

Related products