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Rat TNF-alpha

TNFSF2, Cachectin, DIF, Necrosin, Cytotoxin, Cachexin, TNF

SKU Select size Price Buy
300-18-5ug Mini: 5 ug $60.00  
300-18-20ug Std.: 20 ug $150.00  
300-18-100ug Value: 100 ug $375.00  
300-18-500ug Midgi: 500 ug $1620.00  
300-18-1mg Mg: 1 $2700.00  

Catalog Number: 300-18

Alternate Names:  TNFSF2, Cachectin, DIF, Necrosin, Cytotoxin, Cachexin, TNF               Accession Number: P16599
Description:  Tumor necrosis factor alpha (TNF-α) is an inflammatory cytokine secreted by macrophages, monocytes, neutrophils, T cells, and NK-cells following stimulation by bacterial lipopolysaccharide (LPS). TNF-α signal activation occurs through two receptors, TNFR1 and TNFR2. TNFR1 is expressed on most cell types, unlike TNFR2, which is expressed mainly on immune cells. TNF-α functions to stimulate phagocytosis in macrophages, chemoattract neutrophils, increase insulin resistance, and induce fever.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 17.3 kDa (157 aa)
AA Sequence:  MLRSSSQNSS DKPVAHVVAN HQAEEQLEWL SQRANALLAN GMDLKDNQLV VPADGLYLIY SQVLFKGQGC PDYVLLTHTV SRFATSYQEK VSLLSAIKSP CPKDTPEGAE LKPWYEPMYL GGVSQLEKGD LLSAEVNLPK YLDITESGQV YFGVIAL
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

Cytolysis of mouse L929 cells in the presence of Actinomycin D

≤50 pg/mL; ≥ 2.0 x 10^7 units/mg

(typical ED50 is < 10 pg/mL)

 

 Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature              Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

Data Sheet (PDF)

SDS Document (PDF)

Papers that cite this paper:
Tumor Necrosis Factor ? Decreases Glucagon-Like Peptide-2 Expression by Up-Regulating G-Protein–Coupled Receptor 120 in Crohn Disease

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  • Select size
    Mini: 5 ug, Std.: 20 ug, Value: 100 ug, Midgi: 500 ug, Mg: 1