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Rat IL-6 Induced Proliferation of 7TD1 Cells
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Rat IL-6

Animal-Free (AF) proteins are identical to regular proteins and include additional documentation showing that they were produced with no materials of animal or human origin using ANIMAL-FREE purification processes.  Learn more HERE.

SKU Select size Price Buy
300-09-2ug Mini: 2 ug $63.00 + -
300-09-10ug Std.: 10 ug $158.00 + -
300-09-100ug Value: 100 ug $609.00 + -
300-09-500ug Midgi: 500 ug $3024.00 + -
300-09-1mg Mg: 1 mg $5040.00 + -
300-09AF-2ug Animal-Free Mini: 2 ug $69.00 + -
300-09AF-10ug Animal-Free Std.: 10 ug $174.00 + -
300-09AF-100ug Animal-Free Value: 100 ug $667.00 + -
300-09AF-500ug Animal-Free Midgi: 500 ug $3329.00 + -
300-09AF-1mg Animal-Free Mg: 1 mg $5544.00 + -
  • Weight
    0.0100 lbs
  • SKU
    300-09-2ug
Alternate Names:  IFN-β2, B-cell Differentiation Factor, BSF-2, HSF, MGI-2              
Accession Number: P20607
Description:  Interleukin 6 (IL-6) is a cytokine that is expressed by T cells, macrophages, and muscle cells. IL-6 acts to stimulate an immune response upon infection or trauma. IL-6 has both pro-inflammatory and anti-inflammatory functions, is capable of promoting fever, and signals through the cell-surface type I cytokine receptor complex containing the IL-6Ra and gp130 chains.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 21.9 kDa (188 aa)
AA Sequence:  MFPTSQVRRG DFTEDTTHNR PVYTTSQVGG LITYVLREIL EMRKELCNGN SDCMNSDDAL SENNLKLPEI QRNDGCFQTG YNQEICLLKI CSGLLEFRFY LEFVKNNLQD NKKDKARVIQ SNTETLVHIF KQEIKDSYKI VLPTPTSNAL LMEKLESQKE WLRTKTIQLI LKALEEFLKV TMRSTRQT
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) + 5:1 mannitol to protein

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥90%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

B9 cell proliferation

ED50 ≤ 2 ng/mL (≥ 5.0 x 10^5 units/mg) 

In addition to the acceptance criteria, this product has been tested with the following assay:
(1) 7TD1 cell proliferation (typical ED50 is < 10 pg/mL)

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             
Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile 10 mM HCl at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date                      
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

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Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 1011 Lot 0416
SDS Lot 0413  
PDS Animal-Free Lot 0413 Animal-Free  
SDS Animal-Free    

 

Papers that cite this product:

 

 

Anesth Analg. 2019;128(6):1190‐1198. doi:10.1213/ANE.0000000000004051

Maternal Noninfectious Fever Enhances Cell Proliferation and Microglial Activation in the Neonatal Rat Dentate Gyrus. 

Pancaro C, Boulanger-Bertolus J, Segal S, et al.


 2013 Jan;6(1):136-45. doi: 10.1038/mi.2012.57. Epub 2012 Jul 4.

Protein kinase CK2 is a critical regulator of epithelial homeostasis in chronic intestinal inflammation.