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Rat IFN-gamma

Type II interferon, T cell interferon, Immune Interferon, MAF

SKU Select size Price Buy
300-04-20ug Mini: 20 ug $60.00  
300-04-100ug Std.: 100 ug $150.00  
300-04-250ug Value: 250 ug $345.00  
300-04-500ug Midgi: 500 ug $575.00  
300-04-1mg Mg: 1 mg $1000.00  
View the available lots and COAs for this product HERE

Catalog Number: 300-04

Alternate Names:  Type II interferon, T cell interferon, Immune Interferon, MAF               Accession Number: P01581
Description:  Interferon gamma (IFN-γ) is a type II interferon that is critical during adaptive and innate immune responses to infection.  IFN-γ is produced by T cells and natural killer cells following antigen-specific activation.  IFN-γ binds IFN-γ receptors (IFN-γ R1 and IFN-γ R2), which are expressed on most immune cells, to activate the JAK-STAT pathway.  IFN-γ-induced signaling increases the expression of class 1 major histocompatibility complex (MHC) molecules.  
Source:  Genetically modified E.coli.
Predicted MW: Noncovalently-linked homodimer, 15.6 / 31.2 kDa (135 / 270 aa) 
AA Sequence:  MQGTLIESLE SLKNYFNSSS MDAMEGKSLL LDIWRNWQKD GNTKILESQI ISFYLRLFEV LKDNQAISNN ISVIESHLIT NFFSNSKAKK DAFMSIAKFE VNNPQIQHKA VNELIRVIHQ LSPESSLRKR KRSRC
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 100 mM sodium chloride, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

Bioactive protein, but no acceptance criteria established

≤NA; ≥ Currently undetermined

 

In addition to the acceptance criteria, this product has been tested with the following assay: (1)  Viral CPE assay using EMC virus on L929 cells (first detectable at 9.0 x 10^6 units/mg)

 Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature              Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. If a precipitate is observed, centrifuge the solution thoroughly and use only the soluble fraction (removing it from the precipitate). A 10% overfill has been added to compensate for any loss of protein in the precipitate. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.  

Expiration Date:                      
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

Data Sheet (PDF)

SDS Document (PDF)

Papers that use this product (PMID):
23740952
21885043
23056550
 

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  • Select size
    Mini: 20 ug, Std.: 100 ug, Value: 250 ug, Midgi: 500 ug, Mg: 1 mg