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Mouse FGF-basic (FGF2)

Animal-Free (AF) proteins are identical to regular proteins and include additional documentation showing that they were produced with no materials of animal or human origin using ANIMAL-FREE purification processes.  Learn more HERE.

SKU Select size Price Buy
200-12-10ug Mini: 10 ug $60.00  
200-12-50ug Std.: 50 ug $150.00  
200-12-100ug Value: 100 ug $270.00  
200-12-500ug Midgi: 500 ug $690.00  
200-12-1mg Mg: 1 mg $1200.00  
200-12AF-10ug Animal-Free Mini: 10 ug $65.00  
200-12AF-50ug Animal-Free Std.: 50 ug $165.00  
200-12AF-100ug Animal-Free Value: 100 ug $295.00  
200-12AF-500ug Animal-Free Midgi: 500 ug $760.00  
200-12AF-1mg Animal-Free Mg: 1 mg $1320.00  
View the available lots and COAs for this product HERE

Catalog Number: 200-12 and 200-12AF

Alternate Names:  FGF2, HBGF-2, Prostatropin               Accession Number: P15655
Description:  Basic fibroblast growth factor (FGF-basic), also known as FGF-2, is expressed by endothelial cells and is a mediator of angiogenesis. FGF-basic also has cardioprotective functions during heart injury. The application of FGF-basic is a critical component for human embryonic stem cell culture systems and is necessary for maintaining human embryonic stem cells in an undifferentiated state.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 16.5 kDa (146 aa)
AA Sequence:  MPALPEDGGA AFPPGHFKDP KRLYCKNGGF FLRIHPDGRV DGVREKSDPH VKLQLQAEER GVVSIKGVCA NRYLAMKEDG RLLASKCVTE ECFFFERLES NNYNTYRSRK YSSWYVALKR TGQYKLGSKT GPGQKAILFL PMSAKS
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM sodium chloride, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

3T3 cell proliferation

≤ED50 ≤ 2.5 ng/mL (≥ 4.0 x 10^5 units/mg)

 (typical ED50 is < 0.5 ng/mL)


 
Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature              Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

Data Sheet (PDF)

SDS Document (PDF)

Animal-Free Documents:

Data Sheet AF (PDF)

SDS Document AF (PDF)

Papers that use this product (PMID):
24962614
24025767
21521373
 

To receive more information or a quote, complete our bulk/custom order form.

  • Select size
    Mini: 10 ug, Std.: 50 ug, Value: 100 ug, Midgi: 500 ug, Mg: 1 mg, Animal-Free Mini: 10 ug, Animal-Free Std.: 50 ug, Animal-Free Value: 100 ug, Animal-Free Midgi: 500 ug, Animal-Free Mg: 1 mg