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Mouse EBI3 Subunit (IL-27 / IL-35)
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Mouse EBI3 Subunit (IL-27 / IL-35)

SKU Select size Price Buy
200-57-2ug Mini: 2 ug $60.00  
200-57-10ug Std.: 10 ug $150.00  
200-57-100ug Value: 100 ug $580.00  
200-57-500ug Midgi: 500 ug $2880.00  
200-57-1mg Mg: 1 mg $4800.00  

Catalog Number: 200-57

Alternate Names:  IL-35/EBI3, IL-27 EBI3 subunit, IL-35 EBI3 subunit               Accession Number: O35228
Description:  Epstein-Barr virus induced gene 3 (EBI3) is a secreted glycoprotein belonging to the hematopoietin receptor family related to the p40 subunit of interleukin 12 (IL-12).  EBI3 expression is induced in B-lymphocytes in response to Epstein-Barr virus infection.  EBI3 forms heterodimers with p28 to form interleukin 27 (IL-27), and with p35 to form interleukin 35 (IL-35).  Both IL-27 and IL-35 have anti-inflammatory and regulatory activity.     
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 23 kDa (207 aa)
AA Sequence:  MALVALSQPR VQCHASRYPV AVDCSWTPLQ APNSTRSTSF IATYRLGVAT QQQSQPCLQR SPQASRCTIP DVHLFSTVPY MLNVTAVHPG GASSSLLAFV AERIIKPDPP EGVRLRTAGQ RLQVLWHPPA SWPFPDIFSL KYRLRYRRRG ASHFRQVGPI EATTFTLRNS KPHAKYCIQV SAQDLTDYGK PSDWSLPGQV ESAPHKP
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium citrate

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥90%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

No test available

≤NA; ≥ NA

 Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature              Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws. Upon reconstitution, a small amount of visible precipitate can be expected. A 10% overfill has been added to the total material vialed to compensate for this loss.   

Expiration Date:                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

Data Sheet (PDF)

SDS Document (PDF)

Complementary antibody products:
Need an Anti-Mouse EBI3 antibody?  We recommend:
Anti-Mouse EBI3 (IL-35) Monoclonal Antibodies

Or Anti-Mouse EBI3 Subunit Rabbit Polyclonal Antibody from ImmunoReagents.  If you are curious to know more about the relationship between Shenandoah and Immunoreagents, click here for the scoop.

To receive more information or a quote, complete our bulk/custom order form.

  • Select size
    Mini: 2 ug, Std.: 10 ug, Value: 100 ug, Midgi: 500 ug, Mg: 1 mg