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Mouse EGF

Animal-Free (AF) proteins are identical to regular proteins and include additional documentation that they were produced using ANIMAL-FREE PROCESSES and contain only ANIMAL-FREE RAW MATERIALS.  Learn more HERE.

SKU Select size Price Buy
200-53-100ug Mini: 100 ug $60.00  
200-53-500ug Std.: 500 ug $150.00  
200-53-1mg Value: 1 mg $220.00  
200-53-5mg Mg: 5 mg $1000.00  
200-53AF-100ug Animal-Free Mini: 100 ug $65.00  
200-53AF-500ug Animal-Free Std.: 500 ug $165.00  
200-53AF-1mg Animal-Free Value: 1 mg $240.00  
200-53AF-5mg Animal-Free Mg: 5 mg $1100.00  

Catalog Number: 200-53

Alternate Names:  Urogastrone, URG               Accession Number: P01132
Description:  Epidermal growth factor (EGF) is a growth factor that stimulates the proliferation, differentiation, and survival of epithelial and epidermal cells. EGF contains three intramolecular disulfide bonds and binds in high affinity to the epidermal growth factor receptor (EGFR).
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 6 kDa (53 aa)
AA Sequence:  NSYPGCPSSY DGYCLNGGVC MHIESLDSYT CNCVIGYSGD RCQTRDLRWW ELR
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

3T3 cell proliferation

≤250 pg/mL; ≥ 4.0 x 10^6 units/mg

(typical ED50 is 10-100 pg/mL)

 Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature              Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws. Upon reconstitution, a small amount of visible precipitate can be expected. A 10% overfill has been added to the total material vialed to compensate for this loss.   

Expiration Date:                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

Data Sheet (PDF)

SDS Document (PDF)

Animal-Free Documents:

Data Sheet AF (PDF)

SDS Document AF (PDF)

To receive more information or a quote, complete our bulk/custom order form.

  • Select size
    Mini: 100 ug, Std.: 500 ug, Value: 1 mg, Mg: 5 mg, Animal-Free Mini: 100 ug, Animal-Free Std.: 500 ug, Animal-Free Value: 1 mg, Animal-Free Mg: 5 mg