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Bioassay:  Mouse EGF-Biotin Conjugate Induced Proliferation of 3T3 Cells
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Mouse EGF Biotin Conjugate

* This product will be discontinued on December 31, 2019

 

Alternate Names:  Urogastrone, URG

SKU Select size Price Buy
200-74-5UG Mini: 5 ug $63.00 + -
200-74-20UG Std.: 20 ug $231.00 + -
  • Weight
    0.0100 lbs
  • SKU
    200-74-5UG

Catalog Number: 200-74

Alternate Names:  Urogastrone, URG               Accession Number: P01132
Description:  Epidermal growth factor (EGF) is a growth factor that stimulates the proliferation, differentiation, and survival of epithelial and epidermal cells. EGF contains three intramolecular disulfide bonds and binds in high affinity to the epidermal growth factor receptor (EGFR). The recombinant EGF-Biotin conjugate has an N-terminal leader sequence containing a biotin molecule. There is half of a biotin molecule per EGF protein. The biotin conjugate does not alter the biological activity of the EGF protein.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 7 kDa (61 aa)
AA Sequence:  MKKIDDDKNS YPGCPSSYDG YCLNGGVCMH IESLDSYTCN CVIGYSGDRC QTRDLEWWEL R
Formulation:  In solution: 0.5 mg/mL in sterile PBS

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

3T3 cell proliferation

≤250 pg/mL; ≥ 4.0 x 10^6 units/mg

(typical ED50 is 10-100 pg/mL)

 

Preparation and Storage
Country of Origin:  USA        Shipping:  Ice pack             
Storage:  Centrifuge vial before opening. DO NOT VORTEX. Store at 4°C.
Expiration Date:                      
12 months from date of receipt when stored at 4°C as supplied.

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Product Datasheets Certificates of Analysis
PDS Lot 0314
SDS