Human IL-1 beta

Interleukin 1 beta (IL-1β) is a pro-inflammatory cytokine that is produced by monocytes, macrophages, and dendritic cells (DCs).  IL-1β signals through the IL-1 receptor, type 1 (IL-1R1) to activate the myeloid differentiation primary response 88 (MYD88) signaling pathway, which contains the cytoplasmic Toll/IL-1 receptor (TIR) domain adapter. IL-1β and the independently regulated IL-1α protein have overlapping pro-inflammatory activities to induce adhesion molecule expression on epithelial cells, control fever induction, initiate rheumatoid arthritis, and promote septic shock. 

SKU Select size Price Buy
100-167-2ug Mini: 2 ug $63.00 + -
100-167-10ug Std.: 10 ug $158.00 + -
100-167-100ug Value: 100 ug $457.00 + -
100-167-500ug Midgi: 500 ug $2,268.00 + -
100-167-1mg Mg: 1 mg $3,780.00 + -
100-167AF-2ug Animal-Free Mini: 2 ug $69.00 + -
100-167AF-10ug Animal-Free Std.: 10 ug $174.00 + -
100-167AF-100ug Animal-Free Value: 100 ug $499.00 + -
100-167AF-500ug Animal-Free Midgi: 500 ug $2,494.00 + -
100-167AF-1mg Animal-Free Mg: 1 mg $4,158.00 + -

Alternate Names:  Catabolin, LAF, EP, LEM, MCF               Accession Number: P01584
Description:  Interleukin 1 beta (IL-1β) is a pro-inflammatory cytokine that is produced by monocytes, macrophages, and dendritic cells (DCs).  IL-1β signals through the IL-1 receptor, type 1 (IL-1R1) to activate the myeloid differentiation primary response 88 (MYD88) signaling pathway, which contains the cytoplasmic Toll/IL-1 receptor (TIR) domain adapter. IL-1β and the independently regulated IL-1α protein have overlapping pro-inflammatory activities to induce adhesion molecule expression on epithelial cells, control fever induction, initiate rheumatoid arthritis, and promote septic shock.   

Source:  Genetically modified E.coli.
Predicted MW: Monomer, 17.5 kDa (154 aa)
AA Sequence:  MAPVRSLNCT LRDSQQKSLV MSGPYELKAL HLQGQDMEQQ VVFSMSFVQG EESNDKIPVA LGLKEKNLYL SCVLKDDKPT LQLESVDPKN YPKKKMEKRF VFNKIEINNK LEFESAQFPN WYISTSQAEN MPVFLGGTKG GQDITDFTMQ FVSS
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

D10S cell proliferation

≤12 pg/mL; ≥ 8.3 x 10^7 units/mg

Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature             

Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to -80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

To receive more information or a bulk quote, email us at quotes@shenandoah-bt.com.

 

Product Datasheets Certificates of Analysis Certificates of Origin
PDS Lot 0613 Lot 0613
SDS Lot 0713 Lot 0713
PDS Animal-Free Lot 0613 Animal-Free  
SDS Animal-Free Lot 0713 Animal-Free  

 

Papers that use this product:
 
 
 
 2017 May 5;2(11). pii: eaai7911. doi: 10.1126/sciimmunol.aai7911. PMID 28763790

Platelets subvert T cell immunity against cancer via GARP-TGFβ axis.

Rachidi S1,2Metelli A1,2Riesenberg B1,2Wu BX1,2Nelson MH1,2Wallace C1,2Paulos CM1,2,3Rubinstein MP1,2,3Garrett-Mayer E2,4Hennig M5Bearden DW6Yang Y1,2Liu B1,2Li Z7,2,8.

 2016 Apr 21;11(4):e0153975. doi: 10.1371/journal.pone.0153975. eCollection 2016. PMID 27100888

IL-1 Contributes to the Anti-Cancer Efficacy of Ingenol Mebutate.


 
 

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