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Human FLT-3 Ligand

Animal-Free (AF) proteins are identical to regular proteins and include additional documentation showing that they were produced with no materials of animal or human origin using ANIMAL-FREE purification processes.  Learn more HERE.

SKU Select size Price Buy
100-21-2ug Mini: 2 ug $60.00  
100-21-10ug Std.: 10 ug $150.00  
100-21-100ug Value: 100 ug $435.00  
100-21-500ug Midgi: 500 ug $2160.00  
100-21-1mg Mg: 1 mg $3600.00  
100-21AF-2ug Animal-Free Mini: 2 ug $65.00  
100-21AF-10ug Animal-Free Std.: 10 ug $165.00  
100-21AF-100ug Animal-Free Value: 100 ug $475.00  
100-21AF-500ug Animal-Free Midgi: 500 ug $2375.00  
100-21AF-1mg Animal-Free Mg: 1 mg $3960.00  

Catalog Number: 100-21

Alternate Names:  Flt3L, Fms-related tyrosine kinase 3 ligand               Accession Number: P49771
Description:  Fms-related tyrosine kinase 3 ligand (FLT-3 ligand) is a growth factor that regulates hematopoietic cell proliferation. FLT-3 ligand signalling is transmitted through the fms-related tyrosine kinase 3 (FLT-3) receptor. FLT-3 ligand promotes the long-term expansion and differentiation of pro-B cells in the presence of interleukin 7 (IL-7) or in combination of IL-7 and interleukin 3 (IL-3). Human FLT-3 ligand is active on mouse cells.
Source:  Genetically modified E.coli.
Predicted MW: Monomer, 17.6 kDa (155 aa)
AA Sequence:  MTQDCSFQHS PISSDFAVKI RELSDYLLQD YPVTVASNLQ DEELCGGLWR LVLAQRWMER LKTVAGSKMQ GLLERVNTEI HFVTKCAFQP PPSCLRFVQT NISRLLQETS EQLVALKPWI TRQNFSRCLE LQCQPDSSTL PPPWSPRPLE ATAPT
Formulation:  Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 10 mM sodium phosphate, 50 mM NaCl, pH 7.5

Product Specifications*
*Lot-specific values for the following specifications are supplied with each product on its corresponding COA.  The values provided here are minimum expected values to pass internal requirements. 

Specification

Method of Determination

Acceptance Criteria

Purity

Reducing and Non-Reducing SDS PAGE

≥95%

Endotoxin

Kinetic LAL

≤1 EUs/µg

Biological Activity

OCI-AML5 cell proliferation

≤10 ng/mL; ≥ 1.0 x 10^5 units/mg

(typical ED50 is 1-3 ng/mL)

In addition to the acceptance criteria, this product has been tested with the following assay:
(1)  Production of IL-6 by human PBMCs (typical ED50 is 50-150 ng/mL)

 Preparation and Storage
Country of Origin:  USA        Shipping:  Room temperature              Storage Prior to Reconstitution:  -20°C
Product Reconstitution:  Sterile water at 0.1 mg/mL
Instructions:  Centrifuge vial before opening. Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws.

Expiration Date:                      
12 months from date of receipt when stored at -20°C to-80°C as supplied.
1 month when stored at 4°C after reconstituting as directed.
3 months when stored at -20°C to -80°C after reconstituting as directed.

 Data Sheet (PDF)

SDS Document (PDF)

Animal-Free Documents:

Data Sheet AF (PDF)

SDS Document AF (PDF)

Papers that use this product (PMID):
 

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  • Select size
    Mini: 2 ug, Std.: 10 ug, Value: 100 ug, Midgi: 500 ug, Mg: 1 mg, Animal-Free Mini: 2 ug, Animal-Free Std.: 10 ug, Animal-Free Value: 100 ug, Animal-Free Midgi: 500 ug, Animal-Free Mg: 1 mg