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The Ins and Outs of Shenandoah’s Animal-Free Proteins

Shenandoah is proud to offer our line of Animal-Free Protein products.  These products are provided carrier-free.  No materials of animal or human origin were used to manufacture these proteins and no viral testing is done. 

Animal-Free proteins are ideal for researchers concerned about the ever-present risk of contaminating human proteins or trace growth factors from FBS and animal-derived ingredients typically used in standard recombinant protein production techniques.  Shenandoah takes the necessary steps to ensure that Shenandoah’s Animal-Free Proteins are produced under stringent protocols, with strict standards, and documented accordingly.

Shenandoah’s Animal-Free Production Process:

  • NO FBS or Animal-Derived Ingredients
  • Non-mammalian production system (E.coli-based system)
  • Animal-Free facility, fermenter, columns, filters, labware, lyophilization and storage

Shenandoah’s Animal-Free Quality Standards (*product-specific exceptions are noted when applicable):

  • Endotoxin levels are <1.0 EU per ug protein via LAL (included on lot-specific COA)
  • Purity levels are 95% or greater (included on lot-specific COA)
  • Bioactivity is comparable to recombinant protein produced in traditional techniques (included on lot-specific COA if/when available)

Shenandoah’s Animal-Free Documentation:

  • Certificate of Analysis-  included with each product
  • Certificate of Origin-  available upon request
  • Additional Documentation-  available upon request
  • Amino acid analysis
  • Protein concentration determination
  • Lot-specific SDS-PAGE
  • Lot-specific endotoxin via LAL analysis
  • Lot-specific bioactivity (if available)

Shenandoah’s Animal Free Proteins Common User:

  • Stem cell cultures
  • Serum free or chemically-defined media supplementation
  • Other ex vivo pre-clinical research


These products are for RESEARCH PURPOSES ONLY and are not FDA approved for any use in humans.