Shenandoah's GM-CSF in the news!
Shenandoah's GM-CSF was recently cited in an article from Advanced Materials titled, "SARS-CoV- RBD Neutralizing Antibody Induction is Enhanced by Particulate Vaccination".
Article Authors: Wei-Chiao Huang, Shiqi Zhou, Xuedan He, Kevin Chiem, Moustafa T. Mabrouk, Ruth H. Nissly, Ian M. Bird, Mike Strauss, Suryaprakash Sambhara, Joaquin Ortega, Elizabeth A. Wohlfert, Luis Martinez-Sobrido, Suresh V. Kuchipudi, Bruce A. Davidson, and Jonathan F. Lovell*
The article summary states: "The receptor-binding domain (RBD) of the SARS-CoV-2 spike protein is a candidate vaccine antigen that binds angiotensin-converting enzyme (ACE2), leading to virus entry. Here, it is shown that rapid conversion of recombinant RBD into particulate form via admixing with liposomes containing cobalt-porphyrin-phospholipid (CoPoP) potently enhances the functional antibody response. Antigen binding via His-tag insertion into the CoPoP bilayer results in a serum-stable and conformationally intact display of the RBD on the lipo-some surface. Compared to other vaccine formulations, immunization using CoPoP liposomes admixed with recombinant RBD induces multiple orders of magnitude higher levels of antibody titers in mice that neutralize pseudovirus cell entry, block RBD interaction with ACE2, and inhibit live virus replication. Enhanced immunogenicity can be accounted for by greater RBD uptake into antigen-presenting cells in particulate form and improved immune cell inﬁltration in draining lymph nodes. QS-21 inclusion in the liposomes results in an enhanced antigen-speciﬁc polyfunctional T cell response. In mice, high dose immunization results in minimal local reactogenicity, is well-tolerated, and does not elevate serum cobalt levels. Taken together, these results conﬁrm that particulate presentation strategies for the RBD immunogen should be considered for inducing strongly neutralizing antibody responses against SARS-CoV-2.